Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Orrillo, A.G.; Ledesma, P.; Delgado, O.D.; Spagna, G.; Breccia, J.D.
    Cold-active alpha -L-rhamnosidase from psychrotolerant bacteria isolated from a sub-Antarctic ecosystem (2007), Enzyme Microb. Technol., 40, 236-241.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.40 ethanol presence of 12% ethanol reveals a decreased activity of 48% Pseudoalteromonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.40 intracellular
-
 Ralstonia pickettii 5622
-
3.2.1.40 intracellular
-
 Pseudoalteromonas sp. 5622
-

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Pseudoalteromonas sp.
-
 isolate 005NJ
-
3.2.1.40 Ralstonia pickettii
-
 isolate 009RG
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.40 culture fluid
-
 Ralstonia pickettii
-
3.2.1.40 culture fluid different carbon sources tested Pseudoalteromonas sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.40 additional information
-
 activity analyzed at 4°C and 7°C, 100% activity arbitrarily established as the activity shown at 35°C, corresponds to 151 U/ml in Ralstonia pickettii, activity about 5% at 4°C and about 15% at 7°C determined Ralstonia pickettii
3.2.1.40 additional information
-
 the isolate 005NJ retains 19% alpha-L-rhamnosidase activity at 7°C and 6% activity at 4°C, relative activity depends on carbon source, maximal specific activity obtained at concentrations of 0.5% (w/v) for L-rhamnose (967 U/mg) and apple pectin (382 U/mg) Pseudoalteromonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 p-nitrophenol-alpha-L-rhamnopyranoside + H2O assay reaction system to determine enzyme activity in psychrophile bacteria Ralstonia pickettii p-nitrophenol + alpha-L-rhamnopyranose
-
 ?
3.2.1.40 p-nitrophenol-alpha-L-rhamnopyranoside + H2O assay reaction system to determine enzyme activity in psychrophile bacteria, at 25°C and at 5°C Pseudoalteromonas sp. p-nitrophenol + alpha-L-rhamnopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.40 alpha-L-rhamnosidase
-
 Ralstonia pickettii
3.2.1.40 alpha-L-rhamnosidase
-
 Pseudoalteromonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 40
-
 estimated by activity assay, 6% activity retained at 4°C, half-life of 4 min at 50°C indicates thermo-sensitivity Pseudoalteromonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.40 4 40 activity measured, 6% activity maintained at 4°C, thermo-inactivation above 50°C Pseudoalteromonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 6
-
 estimated by activity assay Pseudoalteromonas sp.