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Literature summary extracted from

  • Schnitzhofer, W.; Weber, H.; Vrsanska, M.; Biely, P.; Cavaco-Paulo, A.; Guebitz, G.M.
    Purification and mechanistic characterization of two polygalacturonases from Sclerotium rolfsii (2007), Enzyme Microb. Technol., 40, 1739-1747.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.15 Ca2+ strongly inhibits PG1 and PG2 Athelia rolfsii
3.2.1.15 Cr3+ stronger inhibitor for PG2 than for PG1 Athelia rolfsii
3.2.1.15 Fe3+ stronger inhibitor for PG1 than for PG2 Athelia rolfsii
3.2.1.15 Hg2+ stronger inhibitor for PG1 than for PG2 Athelia rolfsii
3.2.1.15 Mg2+
-
Athelia rolfsii
3.2.1.15 additional information EDTA and surfactants have no influence on enzyme activity Athelia rolfsii
3.2.1.15 SDS stronger inhibitor for PG1 than for PG2 Athelia rolfsii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.15 38000
-
PG2 Athelia rolfsii
3.2.1.15 39500
-
PG1 Athelia rolfsii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Athelia rolfsii
-
-
-
3.2.1.15 Athelia rolfsii CBS 350.80
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 to homogeneity, by free flow electrophoresis in isoelectric focusing mode using precipitation, followed by gel filtration Athelia rolfsii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 additional information no activity of PG1 and PG2 towards xylan, carboxymethylcellulose and mannan Athelia rolfsii ?
-
?
3.2.1.15 additional information no activity of PG1 and PG2 towards xylan, carboxymethylcellulose and mannan Athelia rolfsii CBS 350.80 ?
-
?
3.2.1.15 pectin + H2O citrus pectin with degree of esterification of 63-66%, bad substrate for PG1 and PG2. Apple pectin with degree of esterification of 70-75%, bad substrate for PG1 and PG2 Athelia rolfsii ?
-
?
3.2.1.15 pectin + H2O citrus pectin with degree of esterification of 63-66%, bad substrate for PG1 and PG2. Apple pectin with degree of esterification of 70-75%, bad substrate for PG1 and PG2 Athelia rolfsii CBS 350.80 ?
-
?
3.2.1.15 polygalacturonic acid + H2O PG1 and PG2 Athelia rolfsii ?
-
?
3.2.1.15 polygalacturonic acid + H2O PG1 and PG2 Athelia rolfsii CBS 350.80 ?
-
?
3.2.1.15 [beta-D-GalA(1-4)]3 + H2O PG1 and PG2 Athelia rolfsii ?
-
?
3.2.1.15 [beta-D-GalA(1-4)]3 + H2O PG1 and PG2 Athelia rolfsii CBS 350.80 ?
-
?
3.2.1.15 [beta-D-GalA(1-4)]4 + H2O only cleaved by PG1 and PG2 when trigalacturonic acid is present Athelia rolfsii ?
-
?
3.2.1.15 [beta-D-GalA(1-4)]4 + H2O only cleaved by PG1 and PG2 when trigalacturonic acid is present Athelia rolfsii CBS 350.80 ?
-
?
3.2.1.15 [beta-D-GalA(1-4)]5 + H2O PG1 and PG2 Athelia rolfsii ?
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 PG1
-
Athelia rolfsii
3.2.1.15 PG2
-
Athelia rolfsii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.15 55
-
isoenzyme PG2 Athelia rolfsii
3.2.1.15 60
-
isoenzyme PG1 Athelia rolfsii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.15 additional information
-
PG1 is stable at 50°C, at 70°C stability is drastically reduced. PG2 is a very sensitive enzyme, its half-life time at 50°C is in the range of min and at 70°C the enzyme is inactivated within 1 min Athelia rolfsii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 4.5
-
isoenzyme PG2 Athelia rolfsii
3.2.1.15 5
-
isoenzyme PG1 Athelia rolfsii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.15 5
-
PG1 is stable at pH 5, exhibits more sensitivity against pH values below and above 5 Athelia rolfsii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.15 Athelia rolfsii PG2
-
5.4
3.2.1.15 Athelia rolfsii PG1
-
6.5

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.2.1.15 0.07
-
isoenzyme PG1 Athelia rolfsii Hg2+
3.2.1.15 0.5
-
isoenzyme PG1 Athelia rolfsii SDS
3.2.1.15 0.75
-
isoenzyme PG1 Athelia rolfsii Fe3+
3.2.1.15 0.9
-
isoenzyme PG2 Athelia rolfsii Hg2+
3.2.1.15 5
-
isoenzyme PG1 and PG2 Athelia rolfsii Mg2+
3.2.1.15 7
-
isoenzyme PG1 Athelia rolfsii Ca2+
3.2.1.15 12
-
isoenzyme PG2 Athelia rolfsii SDS
3.2.1.15 15
-
isoenzyme PG2 Athelia rolfsii Ca2+
3.2.1.15 16
-
isoenzyme PG2 Athelia rolfsii Fe3+
3.2.1.15 18
-
isoenzyme PG2 Athelia rolfsii Cr3+
3.2.1.15 32
-
isoenzyme PG1 Athelia rolfsii Cr3+