EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.15 | Ca2+ | strongly inhibits PG1 and PG2 | Athelia rolfsii | |
3.2.1.15 | Cr3+ | stronger inhibitor for PG2 than for PG1 | Athelia rolfsii | |
3.2.1.15 | Fe3+ | stronger inhibitor for PG1 than for PG2 | Athelia rolfsii | |
3.2.1.15 | Hg2+ | stronger inhibitor for PG1 than for PG2 | Athelia rolfsii | |
3.2.1.15 | Mg2+ | - |
Athelia rolfsii | |
3.2.1.15 | additional information | EDTA and surfactants have no influence on enzyme activity | Athelia rolfsii | |
3.2.1.15 | SDS | stronger inhibitor for PG1 than for PG2 | Athelia rolfsii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.15 | 38000 | - |
PG2 | Athelia rolfsii |
3.2.1.15 | 39500 | - |
PG1 | Athelia rolfsii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.15 | Athelia rolfsii | - |
- |
- |
3.2.1.15 | Athelia rolfsii CBS 350.80 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.15 | to homogeneity, by free flow electrophoresis in isoelectric focusing mode using precipitation, followed by gel filtration | Athelia rolfsii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.15 | additional information | no activity of PG1 and PG2 towards xylan, carboxymethylcellulose and mannan | Athelia rolfsii | ? | - |
? | |
3.2.1.15 | additional information | no activity of PG1 and PG2 towards xylan, carboxymethylcellulose and mannan | Athelia rolfsii CBS 350.80 | ? | - |
? | |
3.2.1.15 | pectin + H2O | citrus pectin with degree of esterification of 63-66%, bad substrate for PG1 and PG2. Apple pectin with degree of esterification of 70-75%, bad substrate for PG1 and PG2 | Athelia rolfsii | ? | - |
? | |
3.2.1.15 | pectin + H2O | citrus pectin with degree of esterification of 63-66%, bad substrate for PG1 and PG2. Apple pectin with degree of esterification of 70-75%, bad substrate for PG1 and PG2 | Athelia rolfsii CBS 350.80 | ? | - |
? | |
3.2.1.15 | polygalacturonic acid + H2O | PG1 and PG2 | Athelia rolfsii | ? | - |
? | |
3.2.1.15 | polygalacturonic acid + H2O | PG1 and PG2 | Athelia rolfsii CBS 350.80 | ? | - |
? | |
3.2.1.15 | [beta-D-GalA(1-4)]3 + H2O | PG1 and PG2 | Athelia rolfsii | ? | - |
? | |
3.2.1.15 | [beta-D-GalA(1-4)]3 + H2O | PG1 and PG2 | Athelia rolfsii CBS 350.80 | ? | - |
? | |
3.2.1.15 | [beta-D-GalA(1-4)]4 + H2O | only cleaved by PG1 and PG2 when trigalacturonic acid is present | Athelia rolfsii | ? | - |
? | |
3.2.1.15 | [beta-D-GalA(1-4)]4 + H2O | only cleaved by PG1 and PG2 when trigalacturonic acid is present | Athelia rolfsii CBS 350.80 | ? | - |
? | |
3.2.1.15 | [beta-D-GalA(1-4)]5 + H2O | PG1 and PG2 | Athelia rolfsii | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.15 | PG1 | - |
Athelia rolfsii |
3.2.1.15 | PG2 | - |
Athelia rolfsii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.15 | 55 | - |
isoenzyme PG2 | Athelia rolfsii |
3.2.1.15 | 60 | - |
isoenzyme PG1 | Athelia rolfsii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.15 | additional information | - |
PG1 is stable at 50°C, at 70°C stability is drastically reduced. PG2 is a very sensitive enzyme, its half-life time at 50°C is in the range of min and at 70°C the enzyme is inactivated within 1 min | Athelia rolfsii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.15 | 4.5 | - |
isoenzyme PG2 | Athelia rolfsii |
3.2.1.15 | 5 | - |
isoenzyme PG1 | Athelia rolfsii |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.15 | 5 | - |
PG1 is stable at pH 5, exhibits more sensitivity against pH values below and above 5 | Athelia rolfsii |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.2.1.15 | Athelia rolfsii | PG2 | - |
5.4 |
3.2.1.15 | Athelia rolfsii | PG1 | - |
6.5 |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
3.2.1.15 | 0.07 | - |
isoenzyme PG1 | Athelia rolfsii | Hg2+ | |
3.2.1.15 | 0.5 | - |
isoenzyme PG1 | Athelia rolfsii | SDS | |
3.2.1.15 | 0.75 | - |
isoenzyme PG1 | Athelia rolfsii | Fe3+ | |
3.2.1.15 | 0.9 | - |
isoenzyme PG2 | Athelia rolfsii | Hg2+ | |
3.2.1.15 | 5 | - |
isoenzyme PG1 and PG2 | Athelia rolfsii | Mg2+ | |
3.2.1.15 | 7 | - |
isoenzyme PG1 | Athelia rolfsii | Ca2+ | |
3.2.1.15 | 12 | - |
isoenzyme PG2 | Athelia rolfsii | SDS | |
3.2.1.15 | 15 | - |
isoenzyme PG2 | Athelia rolfsii | Ca2+ | |
3.2.1.15 | 16 | - |
isoenzyme PG2 | Athelia rolfsii | Fe3+ | |
3.2.1.15 | 18 | - |
isoenzyme PG2 | Athelia rolfsii | Cr3+ | |
3.2.1.15 | 32 | - |
isoenzyme PG1 | Athelia rolfsii | Cr3+ |