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Literature summary extracted from

  • Gote, M.M.; Khan, M.I.; Khire, J.M.
    Active site directed chemical modification of alpha-galactosidase from Bacillus stearothermophilus (NCIM 5146): Involvement of lysine, tryptophan and carboxylate residues in catalytic site (2007), Enzyme Microb. Technol., 40, 1312-1320.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.22 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide 31% residual activity at 100 mM, at pH 6.0 Geobacillus stearothermophilus
3.2.1.22 2,4,6-Trinitrobenzenesulfonic acid 20% residual activity at 2 mM, at pH 8.4 Geobacillus stearothermophilus
3.2.1.22 citraconic anhydride 30% residual activity at 5 mM, at pH 7.8 Geobacillus stearothermophilus
3.2.1.22 diethyldicarbonate complete inhibition at 2 mM, at pH 6.0 Geobacillus stearothermophilus
3.2.1.22 N-bromosuccinimide complete inhibition at 0.02 mM, at pH 5.0 Geobacillus stearothermophilus
3.2.1.22 p-chloromercuribenzoate 16% residual activity at 0.01 mM, at pH 5.8 Geobacillus stearothermophilus
3.2.1.22 Woodward's reagent K 21% residual activity at 5 mM, at pH 6.0 Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.22 Geobacillus stearothermophilus
-
-
-
3.2.1.22 Geobacillus stearothermophilus NCIM 5146
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.22
-
 Geobacillus stearothermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Geobacillus stearothermophilus p-nitrophenol + alpha-D-galactose
-
 ?
3.2.1.22 p-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Geobacillus stearothermophilus NCIM 5146 p-nitrophenol + alpha-D-galactose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.22 Alpha-D-galactoside galactohydrolase
-
 Geobacillus stearothermophilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.22 5.5 7.5
-
 Geobacillus stearothermophilus