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Literature summary extracted from

  • Birgisson, H.; Wheat, J.O.; Hreggvidsson, G.O.; Kristjansson, J.K.; Mattiasson, B.
    Immobilization of a recombinant Escherichia coli producing a thermostable alpha -L-rhamnosidase: Creation of a bioreactor for hydrolyses of naringin (2007), Enzyme Microb. Technol., 40, 1181-1187.
No PubMed abstract available

Application

EC Number Application Comment Organism
3.2.1.40 nutrition application in fruit juice and wine industry, bioreactor design for production of rhamnose from natural flavinoids such as naringin Thermomicrobia bacterium

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.40 expressed in Escherichia coli BL21-DE3 pRIL cells, recombinant strain of Echerichia coli carrying the rhmA gene from Thermomicrobia bacterium, immobilization on Ca2+ alginate beads and packaging the beads onto a column to design a bioreactor for production of rhamnose from naringin Thermomicrobia bacterium

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.40 Thermomicrobia bacterium Q6RCI9 gene rhmA, alpha-L-rhamnosidase A; PRI-1686
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.40 gel filtration Thermomicrobia bacterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.40 cell culture recombinant Echerichia coli cells carrying the rhmA gene of Thermomicrobia bacterium Thermomicrobia bacterium
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.40 additional information
-
 activity measured under different conditions of pH and temperature, free cells and immobilized cells compared to defined conditions of large scale production of rhamnose, assay described, full degradation of 7.9 mM naringin by running the reactor at 1 ml per min at 50°C, optimal flow rates estimated, after four days substrate degradation capacity decreased by 10 to 15% Thermomicrobia bacterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.40 naringin + H2O bitter flavour source in fruit, design of a bioreactor producing alpha-L-rhamnose Thermomicrobia bacterium 4',5,7-trihydroxyflavanone-7-beta-D-glucoside + alpha-L-rhamnose
-
 ?
3.2.1.40 p-nitrophenyl-alpha-L-rhamnopyranoside + H2O assay reaction system to determine enzyme activity in small and large experiments for bioreactor efficiency Thermomicrobia bacterium p-nitrophenol + alpha-L-rhamnopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.40 alpha-L-rhamnosidase
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 Thermomicrobia bacterium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.40 50
-
 optimal temperature for the flow rate of the developed bioreactor over a time of three days, optimal temperature for stability of cells immobilized on beads, full degradation of naringin measured Thermomicrobia bacterium
3.2.1.40 60
-
 alginate immobilized cells and free cells, optimal running temperature of the developed bioreactor over a time of 60 min Thermomicrobia bacterium

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.40 50 70 activity measured, flow rates of bioreactor determined Thermomicrobia bacterium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.40 7.8
-
 optimal pH of enzyme activity using cells immobilized in alginate beads Thermomicrobia bacterium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.40 5.5 9.5 activity measured, no enzyme activity at pH 4.3 of cells on beads in contrast to measured activity of free cells Thermomicrobia bacterium