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Literature summary extracted from
- Characterization of an extracellular poly(3-hydroxyoctanoate) depolymerase from the marine isolate, Pseudomonas luteola M13-4 (2006), Enzyme Microb. Technol., 38, 529-535.
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.76 | Acetic anhydride | 10 mM, 83% inhibition | Pseudomonas luteola |  |
| 3.1.1.76 | dithiothreitol | 10 mM, 12% inhibition | Pseudomonas luteola | |
| 3.1.1.76 | EDTA | 1 mM, 57% inhibition | Pseudomonas luteola | |
| 3.1.1.76 | N-bromosuccinimide | 10 mM, 84% inhibition | Pseudomonas luteola | |
| 3.1.1.76 | NEM | 10 mM, 29% inhibition | Pseudomonas luteola | |
| 3.1.1.76 | phenylmethylsulfonyl fluoride | 10 mM, 93% inhibition | Pseudomonas luteola | |
| 3.1.1.76 | Triton X-100 | 0.1%, 80% inhibition | Pseudomonas luteola | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.1.76 | extracellular | - |
Pseudomonas luteola | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.76 | 28000 | - |
gel filtration | Pseudomonas luteola |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.76 | Pseudomonas luteola | - |
- |
- |
| 3.1.1.76 | Pseudomonas luteola M13-4 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.76 | - |
Pseudomonas luteola |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.76 | 4-nitrophenyl dodecanoate + H2O | 10.4% of the activity with p-nitrophenydecanoate | Pseudomonas luteola | 4-nitrophenol + dodecanoate | - |
? | |
| 3.1.1.76 | 4-nitrophenyl dodecanoate + H2O | 10.4% of the activity with p-nitrophenydecanoate | Pseudomonas luteola M13-4 | 4-nitrophenol + dodecanoate | - |
? | |
| 3.1.1.76 | 4-nitrophenyl hexanoate + H2O | 58.4% of the activity with p-nitrophenydecanoate | Pseudomonas luteola | 4-nitrophenol + hexanoate | - |
? | |
| 3.1.1.76 | 4-nitrophenyl hexanoate + H2O | 58.4% of the activity with p-nitrophenydecanoate | Pseudomonas luteola M13-4 | 4-nitrophenol + hexanoate | - |
? | |
| 3.1.1.76 | 4-nitrophenyl octanoate + H2O | 71.9% of the activity with p-nitrophenydecanoate | Pseudomonas luteola | 4-nitrophenol + octanoate | - |
? | |
| 3.1.1.76 | 4-nitrophenyl octanoate + H2O | 71.9% of the activity with p-nitrophenydecanoate | Pseudomonas luteola M13-4 | 4-nitrophenol + octanoate | - |
? | |
| 3.1.1.76 | additional information | no activity with polycaprolactone and poly(3-hydroxybutyrate) | Pseudomonas luteola | ? | - |
? | |
| 3.1.1.76 | additional information | no activity with polycaprolactone and poly(3-hydroxybutyrate) | Pseudomonas luteola M13-4 | ? | - |
? | |
| 3.1.1.76 | p-nitrophenyl butyrate + H2O | 1.36% of the activity with p-nitrophenydecanoate | Pseudomonas luteola | 4-nitrophenol + butyrate | - |
? | |
| 3.1.1.76 | p-nitrophenyl decanoate + H2O | - |
Pseudomonas luteola | 4-nitrophenol + decanoate | - |
? | |
| 3.1.1.76 | p-nitrophenyl decanoate + H2O | - |
Pseudomonas luteola M13-4 | 4-nitrophenol + decanoate | - |
? | |
| 3.1.1.76 | PHB-co-PHV (60%) + H2O | 74% of activity with poly(3-hydroxyoctanoate) | Pseudomonas luteola | ? | - |
? | |
| 3.1.1.76 | poly(3-hydroxyheptanoate) + H2O | 28.7% of activity with poly(3-hydroxyoctanoate) | Pseudomonas luteola | ? | - |
? | |
| 3.1.1.76 | poly(3-hydroxynonanoate) + H2O | 13.4% of activity with poly(3-hydroxyoctanoate) | Pseudomonas luteola | ? | - |
? | |
| 3.1.1.76 | poly(3-hydroxyoctanoate) + H2O | - |
Pseudomonas luteola | ? | - |
? | |
| 3.1.1.76 | poly(3-hydroxyphenylvalerate) + H2O | 4.1% of activity with poly(3-hydroxyoctanoate) | Pseudomonas luteola | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.76 | monomer | 1 * 28000, SDS-PAGE | Pseudomonas luteola |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.76 | (PHO) depolymerase | - |
Pseudomonas luteola |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.76 | 40 | - |
- |
Pseudomonas luteola |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.76 | 25 | 50 | 25°C: about 65% of maximal activity, 50°C: about 70% of maximal activity | Pseudomonas luteola |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.76 | 10 | - |
- |
Pseudomonas luteola |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.76 | 8.5 | 10.5 | pH 8.5: about 40% of maximal activity, pH 10.5: about 90% of maximal activity | Pseudomonas luteola |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.1.76 | Pseudomonas luteola | isoelectric focusing | - |
6 |
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