EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
5.6.2.2 | adenosine 5'-O-(3-thiotriphosphate) | nonhydrolyzable ATP analogue sustains production of positive supercoils, but to a lesser extent, suggesting that ATP hydrolysis is necessary for efficient activity | Sulfolobus acidocaldarius | |
5.6.2.2 | ATP | 0.005-0.01 mM are sufficient to support full positive supercoiling | Sulfolobus acidocaldarius | |
5.6.2.2 | Polyethylene glycol | in presence of PEG 6000 the reaction becomes very fast and highly processive | Sulfolobus acidocaldarius |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
5.6.2.2 | K+ | ATP-dependent positive supercoiling is optimal at high temperature 75°C in the presence of 5-10 mM Mg2+ and 20-60 mM K+ | Sulfolobus acidocaldarius | |
5.6.2.2 | Mg2+ | ATP-dependent positive supercoiling is optimal at high temperature 75°C in the presence of 5-10 mM Mg2+ and 20-60 mM K+ | Sulfolobus acidocaldarius |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
5.6.2.2 | 13200 | - |
glycerol gradient sedimentation | Sulfolobus acidocaldarius |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.6.2.2 | Sulfolobus acidocaldarius | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.6.2.2 | - |
Sulfolobus acidocaldarius |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.6.2.2 | negatively supercoiled DNA + ATP + H2O | the enzyme catalyzes positive supercoiling either from negatively supercoiled, or from relaxed DNA | Sulfolobus acidocaldarius | positively supercoiled DNA + ADP + phosphate | - |
? | |
5.6.2.2 | relaxed DNA + ATP + H2O | the enzyme catalyzes positive supercoiling either from negatively supercoiled, or from relaxed DNA | Sulfolobus acidocaldarius | positively supercoiled DNA + ADP + phosphate | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.6.2.2 | 75 | - |
ATP-dependent positive supercoiling | Sulfolobus acidocaldarius |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.6.2.2 | 7.9 | - |
assay at | Sulfolobus acidocaldarius |