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Literature summary extracted from

  • Kaur, J.; Chadha, B.S.; Kumar, B.A.; Kaur, G.S.; Saini, H.S.
    Purification and characterization of beta-glucosidase from Melanocarpus sp. MTCC 3922 (2007), Electron. J. Biotechnol., 10, 260-270.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 2-mercaptoethanol
-
 Melanocarpus sp.
3.2.1.21 DTT
-
 Melanocarpus sp.
3.2.1.21 ethanol
-
 Melanocarpus sp.
3.2.1.21 methanol
-
 Melanocarpus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Cu2+
-
 Melanocarpus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 3.3
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C Melanocarpus sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Ca2+ activates Melanocarpus sp.
3.2.1.21 K+ activates Melanocarpus sp.
3.2.1.21 Mg2+ activates Melanocarpus sp.
3.2.1.21 Na+ activates Melanocarpus sp.
3.2.1.21 Zn2+ activates Melanocarpus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 92000
-
 1 * 92000, SDS-PAGE Melanocarpus sp.
3.2.1.21 102000
-
 gel filtration Melanocarpus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Melanocarpus sp.
-
 thermophilic fungus
-
3.2.1.21 Melanocarpus sp. MTCC 3922
-
 thermophilic fungus
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 native enzyme to homogeneity Melanocarpus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-cellobioside + 2 H2O low activity Melanocarpus sp. 4-nitrophenol + 2 beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-cellobioside + 2 H2O low activity Melanocarpus sp. MTCC 3922 4-nitrophenol + 2 beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Melanocarpus sp. 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Melanocarpus sp. MTCC 3922 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 additional information the enzyme exhibits transglycosylation activity with methanol as acceptor Melanocarpus sp. ?
-
 ?
3.2.1.21 additional information the enzyme exhibits transglycosylation activity with methanol as acceptor Melanocarpus sp. MTCC 3922 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 92000, SDS-PAGE Melanocarpus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 60
-
-
 Melanocarpus sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 25 65
-
 Melanocarpus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 50
-
 purified enzyme, stable Melanocarpus sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 66.67
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.0, 60°C Melanocarpus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6
-
-
 Melanocarpus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 5 6 purified enzyme, stable Melanocarpus sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.21 Melanocarpus sp.
-
-
 4.1