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Literature summary extracted from
- Substrate specificity of carboxylesterase isozymes and their contribution to hydrolase activity in human liver and small intestine (2006), Drug Metab. Dispos., 34, 1734-1741.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.1.1 | 0.11 | - |
4-aminobenzoic acid butyl ester | isoform hCE2, 37°C | Homo sapiens |  |
| 3.1.1.1 | 0.13 | - |
4-aminobenzoic acid butyl ester | isoform hCE1, 37°C | Homo sapiens | |
| 3.1.1.1 | 0.322 | - |
4-aminobenzoic acid propyl ester | isoform hCE1, 37°C | Homo sapiens | |
| 3.1.1.1 | 0.356 | - |
4-aminobenzoic acid ethyl ester | isoform hCE1, 37°C | Homo sapiens | |
| 3.1.1.1 | 0.398 | - |
4-aminobenzoic acid propyl ester | isoform hCE2, 37°C | Homo sapiens | |
| 3.1.1.1 | 1.198 | - |
4-aminobenzoic acid ethyl ester | isoform hCE2, 37°C | Homo sapiens | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.1 | Homo sapiens | - |
isoforms hCE1, hCE2 | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.1 | intestine | presence of both isoforms hCE1, hCE2. Dominant isoform is hCE2 | Homo sapiens | - |
| 3.1.1.1 | liver | presence of both isoforms hCE1, hCE2. Dominant isoform is hCE1 | Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.1 | 4-aminobenzoic acid butyl ester + H2O | - |
Homo sapiens | 4-aminobenzoic acid + butanol | - |
? | |
| 3.1.1.1 | 4-aminobenzoic acid ethyl ester + H2O | - |
Homo sapiens | 4-aminobenzoic acid + ethanol | - |
? | |
| 3.1.1.1 | 4-aminobenzoic acid propyl ester + H2O | - |
Homo sapiens | 4-aminobenzoic acid + propanol | - |
? | |
| 3.1.1.1 | 4-nitrobenzoic acid methyl ester + H2O | ratio of the rates of hydrolysis in liver to intestine 5.5 | Homo sapiens | 4-nitrobenzoic acid + methanol | - |
? | |
| 3.1.1.1 | 4-nitrophenyl acetate + H2O | ratio of the rates of hydrolysis in liver to intestine 2.1 | Homo sapiens | 4-nitrophenol + acetate | - |
? | |
| 3.1.1.1 | acetylsalicylic acid + H2O | ratio of the rates of hydrolysis in liver to intestine 0.94 | Homo sapiens | salicylic acid + acetate | - |
? | |
| 3.1.1.1 | betamethasone valerate + H2O | ratio of the rates of hydrolysis in liver to intestine 0.21 | Homo sapiens | betamethasone + valerate | - |
? | |
| 3.1.1.1 | methyl salicylate + H2O | ratio of the rates of hydrolysis in liver to intestine 15.3 | Homo sapiens | salicylic acid + methanol | - |
? | |
| 3.1.1.1 | additional information | isoform hCE2 does not easily form acylated intermediates. The determining factor for the rate of hydrolysis of 4-aminobenzoic acid esters is Km for isoform hCE2, Vmax for isoform hCE1. hCE1 in addition has transesterification abilities | Homo sapiens | ? | - |
? | |
| 3.1.1.1 | temocapril + H2O | ratio of the rates of hydrolysis in liver to intestine 734 | Homo sapiens | (2S)-2-[[(2S,6R)-4-(carboxymethyl)-5-oxo-2-(thiophen-2-yl)-1,4-thiazepan-6-yl]amino]-4-phenylbutanoic acid + ethanol | - |
? | |
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