BRENDA - Enzyme Database

Substrate specificity of carboxylesterase isozymes and their contribution to hydrolase activity in human liver and small intestine

Imai, T.; Taketani, M.; Shii, M.; Hosokawa, M.; Chiba, K.; Drug Metab. Dispos. 34, 1734-1741 (2006) View publication on PubMed

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.1
0.11
-
4-aminobenzoic acid butyl ester
isoform hCE2, 37°C
Homo sapiens
3.1.1.1
0.13
-
4-aminobenzoic acid butyl ester
isoform hCE1, 37°C
Homo sapiens
3.1.1.1
0.322
-
4-aminobenzoic acid propyl ester
isoform hCE1, 37°C
Homo sapiens
3.1.1.1
0.356
-
4-aminobenzoic acid ethyl ester
isoform hCE1, 37°C
Homo sapiens
3.1.1.1
0.398
-
4-aminobenzoic acid propyl ester
isoform hCE2, 37°C
Homo sapiens
3.1.1.1
1.198
-
4-aminobenzoic acid ethyl ester
isoform hCE2, 37°C
Homo sapiens
Organism
EC Number
Organism
UniProt
Commentary
Textmining
3.1.1.1
Homo sapiens
-
isoforms hCE1, hCE2
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.1
intestine
presence of both isoforms hCE1, hCE2. Dominant isoform is hCE2
Homo sapiens
-
3.1.1.1
liver
presence of both isoforms hCE1, hCE2. Dominant isoform is hCE1
Homo sapiens
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
3.1.1.1
4-aminobenzoic acid butyl ester + H2O
-
679473
Homo sapiens
4-aminobenzoic acid + butanol
-
-
-
?
3.1.1.1
4-aminobenzoic acid ethyl ester + H2O
-
679473
Homo sapiens
4-aminobenzoic acid + ethanol
-
-
-
?
3.1.1.1
4-aminobenzoic acid propyl ester + H2O
-
679473
Homo sapiens
4-aminobenzoic acid + propanol
-
-
-
?
3.1.1.1
4-nitrobenzoic acid methyl ester + H2O
ratio of the rates of hydrolysis in liver to intestine 5.5
679473
Homo sapiens
4-nitrobenzoic acid + methanol
-
-
-
?
3.1.1.1
4-nitrophenyl acetate + H2O
ratio of the rates of hydrolysis in liver to intestine 2.1
679473
Homo sapiens
4-nitrophenol + acetate
-
-
-
?
3.1.1.1
acetylsalicylic acid + H2O
ratio of the rates of hydrolysis in liver to intestine 0.94
679473
Homo sapiens
salicylic acid + acetate
-
-
-
?
3.1.1.1
betamethasone valerate + H2O
ratio of the rates of hydrolysis in liver to intestine 0.21
679473
Homo sapiens
betamethasone + valerate
-
-
-
?
3.1.1.1
methyl salicylate + H2O
ratio of the rates of hydrolysis in liver to intestine 15.3
679473
Homo sapiens
salicylic acid + methanol
-
-
-
?
3.1.1.1
additional information
isoform hCE2 does not easily form acylated intermediates. The determining factor for the rate of hydrolysis of 4-aminobenzoic acid esters is Km for isoform hCE2, Vmax for isoform hCE1. hCE1 in addition has transesterification abilities
679473
Homo sapiens
?
-
-
-
?
3.1.1.1
temocapril + H2O
ratio of the rates of hydrolysis in liver to intestine 734
679473
Homo sapiens
(2S)-2-[[(2S,6R)-4-(carboxymethyl)-5-oxo-2-(thiophen-2-yl)-1,4-thiazepan-6-yl]amino]-4-phenylbutanoic acid + ethanol
-
-
-
?
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.1.1.1
0.11
-
4-aminobenzoic acid butyl ester
isoform hCE2, 37°C
Homo sapiens
3.1.1.1
0.13
-
4-aminobenzoic acid butyl ester
isoform hCE1, 37°C
Homo sapiens
3.1.1.1
0.322
-
4-aminobenzoic acid propyl ester
isoform hCE1, 37°C
Homo sapiens
3.1.1.1
0.356
-
4-aminobenzoic acid ethyl ester
isoform hCE1, 37°C
Homo sapiens
3.1.1.1
0.398
-
4-aminobenzoic acid propyl ester
isoform hCE2, 37°C
Homo sapiens
3.1.1.1
1.198
-
4-aminobenzoic acid ethyl ester
isoform hCE2, 37°C
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.1.1.1
intestine
presence of both isoforms hCE1, hCE2. Dominant isoform is hCE2
Homo sapiens
-
3.1.1.1
liver
presence of both isoforms hCE1, hCE2. Dominant isoform is hCE1
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
3.1.1.1
4-aminobenzoic acid butyl ester + H2O
-
679473
Homo sapiens
4-aminobenzoic acid + butanol
-
-
-
?
3.1.1.1
4-aminobenzoic acid ethyl ester + H2O
-
679473
Homo sapiens
4-aminobenzoic acid + ethanol
-
-
-
?
3.1.1.1
4-aminobenzoic acid propyl ester + H2O
-
679473
Homo sapiens
4-aminobenzoic acid + propanol
-
-
-
?
3.1.1.1
4-nitrobenzoic acid methyl ester + H2O
ratio of the rates of hydrolysis in liver to intestine 5.5
679473
Homo sapiens
4-nitrobenzoic acid + methanol
-
-
-
?
3.1.1.1
4-nitrophenyl acetate + H2O
ratio of the rates of hydrolysis in liver to intestine 2.1
679473
Homo sapiens
4-nitrophenol + acetate
-
-
-
?
3.1.1.1
acetylsalicylic acid + H2O
ratio of the rates of hydrolysis in liver to intestine 0.94
679473
Homo sapiens
salicylic acid + acetate
-
-
-
?
3.1.1.1
betamethasone valerate + H2O
ratio of the rates of hydrolysis in liver to intestine 0.21
679473
Homo sapiens
betamethasone + valerate
-
-
-
?
3.1.1.1
methyl salicylate + H2O
ratio of the rates of hydrolysis in liver to intestine 15.3
679473
Homo sapiens
salicylic acid + methanol
-
-
-
?
3.1.1.1
additional information
isoform hCE2 does not easily form acylated intermediates. The determining factor for the rate of hydrolysis of 4-aminobenzoic acid esters is Km for isoform hCE2, Vmax for isoform hCE1. hCE1 in addition has transesterification abilities
679473
Homo sapiens
?
-
-
-
?
3.1.1.1
temocapril + H2O
ratio of the rates of hydrolysis in liver to intestine 734
679473
Homo sapiens
(2S)-2-[[(2S,6R)-4-(carboxymethyl)-5-oxo-2-(thiophen-2-yl)-1,4-thiazepan-6-yl]amino]-4-phenylbutanoic acid + ethanol
-
-
-
?