BRENDA - Enzyme Database

A high throughput screening approach to identify isocitrate lyase inhibitors from traditional Chinese medicine sources

Bai, B.; Xie, J.; Yan, J.; Wang, H.; Hu, C.; Drug Dev. Res. 67, 818-823 (2006)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli strain JM 109
Mycobacterium tuberculosis
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
itaconic anhydride
-
Mycobacterium tuberculosis
4.1.3.1
XHD-1
extract of Illicium verum
Mycobacterium tuberculosis
4.1.3.1
XHD-2
extract of Zingiber officinale
Mycobacterium tuberculosis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.159
-
isocitrate
in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C
Mycobacterium tuberculosis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Mycobacterium tuberculosis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
Ni-Sepharose chromatography
Mycobacterium tuberculosis
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
4°C, 50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0, 2 months, no loss of activity
Mycobacterium tuberculosis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
679472
Mycobacterium tuberculosis
succinate + glyoxylate
-
-
-
?
IC50 Value
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.1.3.1
0.0033
-
in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C
Mycobacterium tuberculosis
itaconic anhydride
4.1.3.1
0.0182
-
in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C
Mycobacterium tuberculosis
XHD-2
4.1.3.1
0.0477
-
in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C
Mycobacterium tuberculosis
XHD-1
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
expressed in Escherichia coli strain JM 109
Mycobacterium tuberculosis
IC50 Value (protein specific)
EC Number
IC50 Value
IC50 Value Maximum
Commentary
Organism
Inhibitor
Structure
4.1.3.1
0.0033
-
in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C
Mycobacterium tuberculosis
itaconic anhydride
4.1.3.1
0.0182
-
in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C
Mycobacterium tuberculosis
XHD-2
4.1.3.1
0.0477
-
in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C
Mycobacterium tuberculosis
XHD-1
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
itaconic anhydride
-
Mycobacterium tuberculosis
4.1.3.1
XHD-1
extract of Illicium verum
Mycobacterium tuberculosis
4.1.3.1
XHD-2
extract of Zingiber officinale
Mycobacterium tuberculosis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
0.159
-
isocitrate
in 50 mM potassium phosphate, 4 mM MgCl2, 4 mM phenylhydrazine, 12 mM cysteine, pH 7.0, at 37°C
Mycobacterium tuberculosis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
Ni-Sepharose chromatography
Mycobacterium tuberculosis
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
4°C, 50 mM NaH2PO4, 300 mM NaCl, 10 mM imidazole, pH 8.0, 2 months, no loss of activity
Mycobacterium tuberculosis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
679472
Mycobacterium tuberculosis
succinate + glyoxylate
-
-
-
?