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Literature summary extracted from
- Substrate specificities and functional characterization of a thermo-tolerant uracil DNA glycosylase (UdgB) from Mycobacterium tuberculosis (2007), DNA Repair, 6, 1517-1528.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.2.15 | overexpression in Escherichia coli | Mycobacterium tuberculosis |
| 3.2.2.28 | gene Rv1259, DNA and amino aid sequence determination and analysis, cloning and overexpression of His6-tagged wild-type and mutant enzymes in Escherichia coli strains | Mycobacterium tuberculosis |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.2.2.28 | additional information | construction of mutants by insertion of kanamycin resistance marker in the UdgB active site motif GQDPY, phenotypes, overview | Mycobacterium tuberculosis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.2.15 | dsDNA | AP-site containing | Mycobacterium tuberculosis | |
| 3.2.2.28 | additional information | UdgB inhibition by dsDNA containing AP-site in the uracil-containing single-nucleotide bulge, UgdB is insensitive against UDG inhibition protein from Bacillus subtilis and shows no inhibition by uracil | Mycobacterium tuberculosis |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.2.15 | Iron | the enzyme contains Fe-S cluster | Mycobacterium tuberculosis |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.2.15 | Mycobacterium tuberculosis | - |
- |
- |
| 3.2.2.28 | Mycobacterium tuberculosis | A5U6Y7 | gene Rv1259 encoding UdgB | - |
| 3.2.2.28 | Mycobacterium tuberculosis H37Ra / ATCC 25177 | A5U6Y7 | gene Rv1259 encoding UdgB | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.2.15 | - |
Mycobacterium tuberculosis |
| 3.2.2.28 | recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strains by nickel affinity chromatography | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.15 | dsDNA + H2O | - |
Mycobacterium tuberculosis | ethenocytosine + ? | - |
? | |
| 3.2.2.15 | dsDNA + H2O | efficiency of its excision is poor from Hx:T or Hx:C pair but better from an Hx:G pair | Mycobacterium tuberculosis | hypoxanthine + ? | - |
? | |
| 3.2.2.15 | dsDNA + H2O | no activity with ssDNA | Mycobacterium tuberculosis | uracil + ? | - |
? | |
| 3.2.2.28 | additional information | UdgB substrate specificity with diverse DNA oligomers, overview, the enzyme excises ethenocytosine and hypoxanthine from dsDNA, in addition to uracil present as a single-nucleotide bulge in dsDNA, but excision of 5-OH-C, dihydroxyuracil, and epsilonA is undetectable, MtuUdgB does not excise uracil from SSU9 ssDNA, mechanism of action, overview | Mycobacterium tuberculosis | ? | - |
? | |
| 3.2.2.28 | additional information | UdgB substrate specificity with diverse DNA oligomers, overview, the enzyme excises ethenocytosine and hypoxanthine from dsDNA, in addition to uracil present as a single-nucleotide bulge in dsDNA, but excision of 5-OH-C, dihydroxyuracil, and epsilonA is undetectable, MtuUdgB does not excise uracil from SSU9 ssDNA, mechanism of action, overview | Mycobacterium tuberculosis H37Ra / ATCC 25177 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.2.15 | UdgB | - |
Mycobacterium tuberculosis |
| 3.2.2.15 | uracil DNA glycosylase | - |
Mycobacterium tuberculosis |
| 3.2.2.28 | double stranded DNA specific UDG | - |
Mycobacterium tuberculosis |
| 3.2.2.28 | dsDNA specific UDG | - |
Mycobacterium tuberculosis |
| 3.2.2.28 | More | UdgB is a family 5 UDG | Mycobacterium tuberculosis |
| 3.2.2.28 | UdgB | - |
Mycobacterium tuberculosis |
| 3.2.2.28 | uracil DNA glycosylase | - |
Mycobacterium tuberculosis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.15 | 60 | - |
10 min, stable below | Mycobacterium tuberculosis |
| 3.2.2.28 | 37 | - |
assay at | Mycobacterium tuberculosis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.28 | 60 | - |
purified UdgB, stable up to, MtuUdgB is thermo-tolerant | Mycobacterium tuberculosis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.28 | 7.5 | - |
assay at | Mycobacterium tuberculosis |
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