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Literature summary extracted from

  • Shi, Y.W.; Cui, L.F.; Yuan, J.M.
    Gene cloning, expression, and substrate specificity of an imidase from the strain Pseudomonas putida YZ-26. (2007), Curr. Microbiol., 55, 61-64.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.16 overexpressed in Escherichia coli Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.16 29.2
-
 succinimide
-
 Pseudomonas putida
3.5.2.16 39.1
-
 Maleimide
-
 Pseudomonas putida
3.5.2.16 53.5
-
 hydantoin
-
 Pseudomonas putida
3.5.2.16 74.3
-
 dihydrouracil
-
 Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.16 36000
-
 x * 36000, (His)6-tagged enzyme, SDS-PAGE Pseudomonas putida
3.5.2.16 141000
-
 gel filtration Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.16 Pseudomonas putida Q4JG22
-
-
3.5.2.16 Pseudomonas putida YZ-26 Q4JG22
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.16 recombinant enzyme Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.16 dihydrouracil + H2O
-
 Pseudomonas putida N-carbamoyl-beta-alanine
-
 ?
3.5.2.16 dihydrouracil + H2O
-
 Pseudomonas putida YZ-26 N-carbamoyl-beta-alanine
-
 ?
3.5.2.16 hydantoin + H2O
-
 Pseudomonas putida hydantoic acid monoamide
-
 ?
3.5.2.16 hydantoin + H2O
-
 Pseudomonas putida YZ-26 hydantoic acid monoamide
-
 ?
3.5.2.16 maleimide + H2O
-
 Pseudomonas putida maleic acid monoamide
-
 ?
3.5.2.16 maleimide + H2O
-
 Pseudomonas putida YZ-26 maleic acid monoamide
-
 ?
3.5.2.16 additional information no activity with DL-5-phenylhydantoin and DL-p-hydroxyphenylhydantoin Pseudomonas putida ?
-
 ?
3.5.2.16 additional information no activity with DL-5-phenylhydantoin and DL-p-hydroxyphenylhydantoin Pseudomonas putida YZ-26 ?
-
 ?
3.5.2.16 succinimide + H2O
-
 Pseudomonas putida succinamic acid monoamide
-
 ?
3.5.2.16 succinimide + H2O
-
 Pseudomonas putida YZ-26 succinamic acid monoamide
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.2.16 ? x * 33712.6, calculated from sequence Pseudomonas putida
3.5.2.16 ? x * 36000, (His)6-tagged enzyme, SDS-PAGE Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.16 2.107
-
 hydantoin
-
 Pseudomonas putida
3.5.2.16 2.94
-
 hydantoin
-
 Pseudomonas putida
3.5.2.16 9.14
-
 dihydrouracil
-
 Pseudomonas putida
3.5.2.16 479
-
 Maleimide
-
 Pseudomonas putida
3.5.2.16 1494
-
 succinimide
-
 Pseudomonas putida