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Literature summary extracted from

  • Luo, H.; Huang, H.; Yang, P.; Wang, Y.; Yuan, T.; Wu, N.; Yao, B.; Fan, Y.
    A novel phytase appA from Citrobacter amalonaticus CGMCC 1696: gene cloning and overexpression in Pichia pastoris (2007), Curr. Microbiol., 55, 185-192.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.26 expressed in Pichia pastoris Citrobacter amalonaticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.26 Ag+ significantly inhibited by 1 mM Ag+ Citrobacter amalonaticus
3.1.3.26 Fe2+ significantly inhibited by 1 mM Fe2+ Citrobacter amalonaticus
3.1.3.26 additional information highly resistant to pepsin or trypsin treatment Citrobacter amalonaticus
3.1.3.26 SDS almost complete inhibition by 1 mM SDS Citrobacter amalonaticus
3.1.3.26 Zn2+ significantly inhibited by 1 mM Zn2+ Citrobacter amalonaticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.26 1
-
 myo-inositol hexakisphosphate recombinant enzyme, in 0.1 M sodium acetate buffer (pH 4.5) at 37°C Citrobacter amalonaticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.26 Ca2+ little enhancement of activity at 1 mM Citrobacter amalonaticus
3.1.3.26 Co2+ little enhancement of activity at 1 mM Citrobacter amalonaticus
3.1.3.26 Mg2+ little enhancement of activity at 1 mM Citrobacter amalonaticus
3.1.3.26 Mn2+ little enhancement of activity at 1 mM Citrobacter amalonaticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.26 46300
-
 deduced from amino acid sequence Citrobacter amalonaticus
3.1.3.26 49000
-
 SDS-PAGE, after deglycosylation Citrobacter amalonaticus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Citrobacter amalonaticus Q003Y3
-
-
3.1.3.26 Citrobacter amalonaticus CGMCC 1696 Q003Y3
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.26 glycoprotein
-
 Citrobacter amalonaticus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26 Sephacryl S-200 HR gel filtration Citrobacter amalonaticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.3.26 3548
-
 recombinant enzyme, at 37°C Citrobacter amalonaticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Citrobacter amalonaticus myo-inositol pentakisphosphate + phosphate
-
 ?
3.1.3.26 myo-inositol hexakisphosphate + H2O
-
 Citrobacter amalonaticus CGMCC 1696 myo-inositol pentakisphosphate + phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.3.26 AppA
-
 Citrobacter amalonaticus
3.1.3.26 histidine acid phosphatase
-
 Citrobacter amalonaticus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.26 55
-
-
 Citrobacter amalonaticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 4.5
-
-
 Citrobacter amalonaticus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.3.26 3 10 more than 85% of the total activity is retained after incubation at pH 3.0 to 10.0 for 1 h Citrobacter amalonaticus