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Literature summary extracted from

  • Barrera-Islas, G.A.; Ramos-Valdivia, A.C.; Salgado, L.M.; Ponce-Noyola, T.
    Characterization of a beta-glucosidase produced by a high-specific growth-rate mutant of Cellulomonas flavigena (2007), Curr. Microbiol., 54, 266-270.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.21 additional information mutant strain PN-120 produces a beta-glucosidase that is 10fold more active with a 3fold higher affinity for cellobiose than the corresponding enzyme isolated from the parental strain, the mutation of strain PN-120 affects the catalytic site Cellulomonas flavigena

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 5.25
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.2, 40°C, mutant enzyme Cellulomonas flavigena
3.2.1.21 7.1
-
 4-nitrophenyl-beta-D-glucopyranoside pH 6.2, 40°C, wild-type enzyme Cellulomonas flavigena
3.2.1.21 37
-
 cellobiose pH 6.2, 40°C, mutant enzyme Cellulomonas flavigena
3.2.1.21 114
-
 cellobiose pH 6.2, 40°C, wild-type enzyme Cellulomonas flavigena

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 210000
-
 gel filtration Cellulomonas flavigena

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 cellobiose + H2O Cellulomonas flavigena
-
 2 beta-D-glucose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Cellulomonas flavigena
-
 wild-type strain CDBB531 and mutant strain PN-120
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 native wild-type and mutant enzymes partially by anion exchange chromatography and gel filtration 29fold and 16fold, respectively Cellulomonas flavigena

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.21 additional information mutant strain PN-120 produces a beta-glucosidase that is 16fold more active than the corresponding enzyme isolated from the parental strain Cellulomonas flavigena
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 2.27
-
 partially purified wild-type enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside Cellulomonas flavigena
3.2.1.21 14.1
-
 partially purified mutant enzyme, substrate 4-nitrophenyl-beta-D-glucopyranoside Cellulomonas flavigena

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-methylumbelliferyl-beta-D-glucopyranoside + H2O
-
 Cellulomonas flavigena 4-methylumbelliferol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Cellulomonas flavigena 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Cellulomonas flavigena 2 beta-D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 oligomer three protein bands in SDS-PAGE Cellulomonas flavigena

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 37 40 wild-type and mutant enzymes Cellulomonas flavigena

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 20 70
-
 Cellulomonas flavigena

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6
-
 wild-type and mutant enzymes Cellulomonas flavigena

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 5 8
-
 Cellulomonas flavigena