Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Klomklao, S.; Kishimura, H.; Yabe, M.; Benjakul, S.
    Purification and characterization of two pepsins from the stomach of pectoral rattail (Coryphaenoides pectoralis) (2007), Comp. Biochem. Physiol. B, 147B, 682-689.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.23.1 EDTA 2 mM, 11.64% inhibition Albatrossia pectoralis
3.4.23.1 NaCl 30%, activity of pepsin A decreases by 60-65% Albatrossia pectoralis
3.4.23.1 pepstatin A 0.01 mM, 98.2% inhibition Albatrossia pectoralis
3.4.23.2 EDTA 2 mM, 12.31% inhibition Albatrossia pectoralis
3.4.23.2 NaCl 30%, activity of pepsin B decreases by 60-65% Albatrossia pectoralis
3.4.23.2 pepstatin A 0.01 mM, 96.7% inhibition Albatrossia pectoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.23.1 0.098
-
 hemoglobin pH 3.0, 37°C Albatrossia pectoralis
3.4.23.2 152
-
 hemoglobin pH 3.0, 37°C Albatrossia pectoralis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.23.1 CaCl2 5 mM, 1.39fold activation Albatrossia pectoralis
3.4.23.1 CoCl2 5 mM, 1.34fold activation Albatrossia pectoralis
3.4.23.1 MgCl2 10 mM, 1.24fold activation Albatrossia pectoralis
3.4.23.2 CaCl2 5 mM, 1.13fold activation Albatrossia pectoralis
3.4.23.2 CoCl2 5 mM, 1.17fold activation Albatrossia pectoralis
3.4.23.2 MgCl2 5 mM, 1.13fold activation Albatrossia pectoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.23.1 35000
-
 1 * 35000, SDS-PAGE Albatrossia pectoralis
3.4.23.2 31000
-
 1 * 31000, SDS-PAGE Albatrossia pectoralis

Organism

EC Number Organism UniProt Comment Textmining
3.4.23.1 Albatrossia pectoralis
-
-
-
3.4.23.2 Albatrossia pectoralis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.23.1
-
 Albatrossia pectoralis
3.4.23.2
-
 Albatrossia pectoralis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.23.1 stomach
-
 Albatrossia pectoralis
-
3.4.23.2 stomach
-
 Albatrossia pectoralis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.23.1 additional information
-
 30.71 U/mg protein. One unit is defined as the amount causing an increase of 1.0 in absorbance at 280 nm per min Albatrossia pectoralis
3.4.23.2 additional information
-
 56.14 U/mg protein. One unit is defined as the amount causing an increase of 1.0 in absorbance at 280 nm per min Albatrossia pectoralis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.23.1 Hemoglobin + H2O
-
 Albatrossia pectoralis ?
-
 ?
3.4.23.2 Hemoglobin + H2O
-
 Albatrossia pectoralis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.23.1 monomer 1 * 35000, SDS-PAGE Albatrossia pectoralis
3.4.23.2 monomer 1 * 31000, SDS-PAGE Albatrossia pectoralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.23.1 45
-
-
 Albatrossia pectoralis
3.4.23.2 45
-
-
 Albatrossia pectoralis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.23.1 20 55 20°C: about 40% of maximal activity, 55°C: about 60% of maximal activity Albatrossia pectoralis
3.4.23.2 20 55 20°C: about 35% of maximal activity, 50°C: about 60% of maximal activity, 55°C: about 25% of maximal activity Albatrossia pectoralis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.23.1 50
-
 15 min, about 10% loss of activity Albatrossia pectoralis
3.4.23.1 60
-
 15 min, about 80% loss of activity Albatrossia pectoralis
3.4.23.2 40
-
 15 min, stable below Albatrossia pectoralis
3.4.23.2 50
-
 15 min, about 50% loss of activity Albatrossia pectoralis
3.4.23.2 60
-
 15 min, about 85% loss of activity Albatrossia pectoralis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.23.1 50
-
 hemoglobin pH 3.0, 37°C Albatrossia pectoralis
3.4.23.2 32
-
 hemoglobin pH 3.0, 37°C Albatrossia pectoralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.23.1 3
-
-
 Albatrossia pectoralis
3.4.23.2 3.5
-
-
 Albatrossia pectoralis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.23.1 2.5 4 pH 2.5: about 55% of maximal activity, pH 4.0: about 55% of maximal activity Albatrossia pectoralis
3.4.23.2 2.5 5 pH 2.5: about 20% of maximal activity, pH 3.0: about 70% of maximal activity, pH 4.0: about 50% of maximal activity Albatrossia pectoralis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.23.1 6
-
 room temperature, 30 min, stable below Albatrossia pectoralis
3.4.23.1 8
-
 room temperature, 30 min, about 65% loss of activity Albatrossia pectoralis
3.4.23.2 6
-
 room temperature, 30 min, stable below Albatrossia pectoralis
3.4.23.2 7
-
 room temperature, 30 min, about 20% loss of activity Albatrossia pectoralis
3.4.23.2 8
-
 room temperature, 30 min, about 75% loss of activity Albatrossia pectoralis