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Literature summary extracted from
- Optimization of an enzymatic method for the determination of lysosomal N-acetyl-beta-D-hexosaminidase and beta-glucuronidase in synovial fluid (2006), Clin. Chem. Lab. Med., 44, 933-937.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.52 | diagnostics | a spectrophotometric method for the determination of lysosomally derived N-acetyl-beta-D-hexosaminidase in synovial fluid on a microplate reader is optimized to improve its utility. The assay is sufficiently sensitive for small volumes of synovial fluid, and is useful for the clinical diagnosis of joint diseases | Homo sapiens |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.52 | lysosome | - |
Homo sapiens | 5764 | - |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.52 | Homo sapiens | - |
- |
- |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.52 | synovial fluid | - |
Homo sapiens | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.52 | 4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | - |
Homo sapiens | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? |  |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 4.7 | - |
in a citrate-phosphate buffer | Homo sapiens |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.52 | 3.5 | 5.1 | pH 3.5: about 45% of maximal activity, pH 5.1: about 65% of maximal activity | Homo sapiens |
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Release 2023.1 (January 2023)
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