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Literature summary extracted from

  • Gagnaire, B.; Geffard, O.; Xuereb, B.; Margoum, C.; Garric, J.
    Cholinesterase activities as potential biomarkers: Characterization in two freshwater snails, Potamopyrgus antipodarum (Mollusca, Hydrobiidae, Smith 1889) and Valvata piscinalis (Mollusca, Valvatidae, Mueller 1774) (2008), Chemosphere, 71, 553-560.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.8 environmental protection the enzyme may be employed as a biological indicator for assessing pesticide contamination Potamopyrgus antipodarum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.8 BW284c51 inhibits acetylthiocholinesterase and propionylthiocholinesterase activities, but not butyrylthiocholineesterase activity Potamopyrgus antipodarum
3.1.1.8 BW284c51 inhibits acetylthiocholinesterase, propionylthiocholinesterase and butyrylthiocholineesterase activities Valvata piscinalis
3.1.1.8 chlorpyrifos the enzyme activity is significantly decreased by environmental realistic chlorpyrifos concentrations of 2.86 and 14.2 nM after seven days of contact Potamopyrgus antipodarum
3.1.1.8 eserine
-
 Potamopyrgus antipodarum
3.1.1.8 eserine
-
 Valvata piscinalis
3.1.1.8 iso-ompa inhibits butyrylthiocholineesterase activity Potamopyrgus antipodarum
3.1.1.8 additional information no inhibition by chlorpyrifos Valvata piscinalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.8 0.13
-
 acetylthiocholine pH 7.8 Potamopyrgus antipodarum
3.1.1.8 0.32
-
 propionylthiocholine pH 7.8 Potamopyrgus antipodarum
3.1.1.8 1.31
-
 acetylthiocholine pH 7.8 Valvata piscinalis
3.1.1.8 3.19
-
 propionylthiocholine pH 7.8 Valvata piscinalis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.8 Potamopyrgus antipodarum
-
 a freshwater snail
-
3.1.1.8 Valvata piscinalis
-
 a freshwater snail
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.8 additional information
-
-
 Potamopyrgus antipodarum
3.1.1.8 additional information
-
-
 Valvata piscinalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.8 acetylthiocholine + H2O
-
 Potamopyrgus antipodarum thiocholine + acetate
-
 ?
3.1.1.8 acetylthiocholine + H2O
-
 Valvata piscinalis thiocholine + acetate
-
 ?
3.1.1.8 butyrylthiocholine + H2O low activity Potamopyrgus antipodarum thiocholine + butyrate
-
 ?
3.1.1.8 butyrylthiocholine + H2O low activity Valvata piscinalis thiocholine + butyrate
-
 ?
3.1.1.8 additional information substrate specificity, overview Potamopyrgus antipodarum ?
-
 ?
3.1.1.8 additional information substrate specificity, overview Valvata piscinalis ?
-
 ?
3.1.1.8 propionylthiocholine + H2O
-
 Potamopyrgus antipodarum thiocholine + propionate
-
 ?
3.1.1.8 propionylthiocholine + H2O
-
 Valvata piscinalis thiocholine + propionate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.8 ChE
-
 Potamopyrgus antipodarum
3.1.1.8 ChE
-
 Valvata piscinalis
3.1.1.8 cholinesterase
-
 Potamopyrgus antipodarum
3.1.1.8 cholinesterase
-
 Valvata piscinalis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.8 7.8
-
 assay at Potamopyrgus antipodarum
3.1.1.8 7.8
-
 assay at Valvata piscinalis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.1.1.8 0.000024
-
 pH 7.8, versus butyrylthiocholine Potamopyrgus antipodarum eserine
3.1.1.8 0.000034
-
 pH 7.8, versus acetylthiocholine Potamopyrgus antipodarum eserine
3.1.1.8 0.00139
-
 pH 7.8, versus acetylthiocholine Valvata piscinalis eserine
3.1.1.8 0.0014
-
 pH 7.8, versus butyrylthiocholine Valvata piscinalis eserine
3.1.1.8 0.00529
-
 pH 7.8, versus propionylthiocholine Potamopyrgus antipodarum eserine
3.1.1.8 0.00868
-
 pH 7.8, versus propionylthiocholine Valvata piscinalis eserine
3.1.1.8 0.15
-
 pH 7.8, versus acetylthiocholine Valvata piscinalis BW284c51
3.1.1.8 0.262
-
 pH 7.8, versus propionylthiocholine Valvata piscinalis BW284c51
3.1.1.8 0.29
-
 pH 7.8, versus acetylthiocholine Potamopyrgus antipodarum BW284c51
3.1.1.8 0.387
-
 pH 7.8, versus propionylthiocholine Potamopyrgus antipodarum BW284c51