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Literature summary extracted from

  • Wutor, V.C.; Togo, C.A.; Pletschke, B.I.
    The effect of physico-chemical parameters and chemical compounds on the activity of beta-D-galactosidase (B-GAL), a marker enzyme for indicator microorganisms in water (2007), Chemosphere, 68, 622-627.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.23 environmental protection the presence of coliforms in polluted water is determined enzymatically in situ by directly monitoring the activity of B-GAL through the hydrolysis of the yellow chromogenic subtrate, chlorophenol red beta-D-galactopyranoside, which produces a red chlorophenol red product, assay evaluation, overview Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 Ag+
-
 Escherichia coli
3.2.1.23 Ca2+ activates at 50-100 mg/l, inhibits at concentrations above 200 mg/l Escherichia coli
3.2.1.23 Carbonate
-
 Escherichia coli
3.2.1.23 chloride
-
 Escherichia coli
3.2.1.23 Citric acid slight inhibition at 200 mg/l Escherichia coli
3.2.1.23 Cu2+
-
 Escherichia coli
3.2.1.23 EDTA
-
 Escherichia coli
3.2.1.23 Fe2+ activates at 50-100 mg/l, inhibits at concentrations above 200 mg/l Escherichia coli
3.2.1.23 ferulic acid
-
 Escherichia coli
3.2.1.23 sodium hypochlorite
-
 Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.23 Ca2+ activates at 50-100 mg/l, inhibits at concentrations above 200 mg/l Escherichia coli
3.2.1.23 Fe2+ activates at 50-100 mg/l, inhibits at concentrations above 200 mg/l Escherichia coli
3.2.1.23 Mg2+ activates at 50-200 mg/l Escherichia coli
3.2.1.23 nitrate activates Escherichia coli
3.2.1.23 sulfate activates Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.23 lactose + H2O Escherichia coli
-
 D-glucose + D-galactose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Escherichia coli
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 chlorophenol red beta-D-galactopyranoside + H2O
-
 Escherichia coli chlorophenol red + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Escherichia coli D-glucose + D-galactose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 B-GAL
-
 Escherichia coli
3.2.1.23 beta-D-galactosidase
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.23 35
-
 two optima, 35°C and 55°C Escherichia coli
3.2.1.23 55
-
 two optima, 35°C and 55°C Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.23 20 65
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.23 7.8
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.23 5 11 60% of maximal activity at pH 7.5 and pH 9.0 Escherichia coli