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Literature summary extracted from

  • Tanaka, T.; Yamamoto, T.; Tsukiji, S.; Nagamune, T.
    Site-specific protein modification on living cells catalyzed by Sortase (2008), Chembiochem, 9, 802-807.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.4.22.70 molecular biology a general strategy for the site-specific modification of cell surface proteins with synthetic molecules by using sortase, a transpeptidase from Staphylococcus aureus. The short peptide tag LPETGG is genetically introduced to the C terminus of the target protein, expressed on the cell surface. Subsequent addition of sortase and an N-terminal triglycine-containing probe results in the site-specific labeling of the tagged protein. C-terminal-specific labeling of osteoclast differentiation factor with a biotin- or fluorophore-containing short peptide on the living cell surface. The labeling reaction occurrs efficiently in serum-containing medium, as well as serum-free medium or PBS. The labeled products are detected after incubation for 5 min. In addition, site-specific protein–protein conjugation is successfully demonstrated on a living cell surface by the Sortase-catalyzed reaction. This strategy provides a powerful tool for cell biology and cell surface engineering Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.22.70 expression in Escherichia coli Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.70 Staphylococcus aureus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.70 His6-SrtA Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
3.4.22.70 sortase A
-
 Staphylococcus aureus
3.4.22.70 SrtA
-
 Staphylococcus aureus