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Literature summary extracted from

  • Kobayashi, K.; Kubo, M.; Yoshioka, S.; Kitagawa, T.; Kato, Y.; Asano, Y.; Aono, S.
    Systematic regulation of the enzymatic activity of phenylacetaldoxime dehydratase by exogenous ligands (2006), Chembiochem, 7, 2004-2009.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.8.1.4 amino-terminal His6-tagged H282G OxdB expressed in Escherichia coli Bacillus sp. (in: Bacteria)

Protein Variants

EC Number Protein Variants Comment Organism
4.8.1.4 H282G by site-directed mutagenesis Bacillus sp. (in: Bacteria)
4.8.1.4 additional information the H282G mutant has no enzymatic activity, the enzymatic activity is rescued by imidazole or pyridine derivatives that act as exogenous proximal ligand Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.8.1.4 0.38
-
 (Z)-phenylacetaldoxime H282G mutant in the presence of pyridine Bacillus sp. (in: Bacteria)
4.8.1.4 0.46
-
 (Z)-phenylacetaldoxime H282G mutant in the presence of 4-chloropyridine Bacillus sp. (in: Bacteria)
4.8.1.4 0.47
-
 (Z)-phenylacetaldoxime H282G mutant in the presence of 4-methylimidazole Bacillus sp. (in: Bacteria)
4.8.1.4 0.48
-
 (Z)-phenylacetaldoxime H282G mutant in the presence of 4-acetylpyridine Bacillus sp. (in: Bacteria)
4.8.1.4 0.67
-
 (Z)-phenylacetaldoxime H282G mutant in the presence of 4-methylpyridine Bacillus sp. (in: Bacteria)
4.8.1.4 0.7
-
 (Z)-phenylacetaldoxime wild type Bacillus sp. (in: Bacteria)
4.8.1.4 0.76
-
 (Z)-phenylacetaldoxime H282G mutant in the presence of imidazole Bacillus sp. (in: Bacteria)
4.8.1.4 0.78
-
 (Z)-phenylacetaldoxime H282G mutant in the presence of 1-methylimidazole Bacillus sp. (in: Bacteria)
4.8.1.4 1.1
-
 (Z)-phenylacetaldoxime H282G mutant in the presence of 2-methylimidazole Bacillus sp. (in: Bacteria)
4.8.1.4 1.64
-
 (Z)-phenylacetaldoxime H282G mutant in the presence of 4-methoxypyridine Bacillus sp. (in: Bacteria)
4.8.1.4 2.52
-
 (Z)-phenylacetaldoxime H282G mutant in the presence of 1-vinylimidazole Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
4.8.1.4 Bacillus sp. (in: Bacteria) P82604
-
-
4.8.1.4 Bacillus sp. (in: Bacteria) OxB-1 P82604
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.8.1.4 by Co2+-charged metal-ion-chelating Talon column chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.8.1.4 (Z)-phenylacetaldehyde oxime
-
 Bacillus sp. (in: Bacteria) phenylacetonitrile + H2O
-
 ?
4.8.1.4 (Z)-phenylacetaldehyde oxime
-
 Bacillus sp. (in: Bacteria) OxB-1 phenylacetonitrile + H2O
-
 ?
4.8.1.4 (Z)-phenylacetaldoxime
-
 Bacillus sp. (in: Bacteria) phenylacetonitrile + H2O
-
 ?
4.8.1.4 (Z)-phenylacetaldoxime
-
 Bacillus sp. (in: Bacteria) OxB-1 phenylacetonitrile + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.8.1.4 phenylacetaldoxime dehydratase
-
 Bacillus sp. (in: Bacteria)