EC Number | Crystallization (Comment) | Organism |
---|---|---|
4.3.3.2 | the crystal structure of STR1 in complex with strictosidine is solved at a resolution of 3.0 A | Rauvolfia serpentina |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.3.3.2 | V208A | Val208 plays a critical role in substrate recognition at the indole moiety of strictosidine | Rauvolfia serpentina |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.3.2 | 0.072 | - |
tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 0.136 | - |
6-fluoro-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 0.219 | - |
tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 0.259 | - |
5-fluoro-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 0.281 | - |
5-methyl-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 0.307 | - |
6-methoxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 0.356 | - |
6-fluoro-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 0.393 | - |
6-methyl-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 0.762 | - |
6-methyl-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 0.844 | - |
5-hydroxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 0.962 | - |
6-methoxy-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 1.302 | - |
5-fluoro-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 2.255 | - |
5-hydroxy-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 3.592 | - |
5-methoxy-tryptamine | mutant V208A | Rauvolfia serpentina |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.2 | 3-alpha(S)-strictosidine + H2O | Rauvolfia serpentina | - |
tryptamine + secologanin | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.3.3.2 | Rauvolfia serpentina | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.3.3.2 | 3-alpha(S)-strictosidine + H2O | - |
Rauvolfia serpentina | tryptamine + secologanin | - |
? | |
4.3.3.2 | 5-fluoro-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? | |
4.3.3.2 | 5-hydroxy-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? | |
4.3.3.2 | 5-methoxy-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? | |
4.3.3.2 | 5-methyl-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? | |
4.3.3.2 | 6-fluoro-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? | |
4.3.3.2 | 6-methoxy-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? | |
4.3.3.2 | 6-methyl-tryptamine + H2O | - |
Rauvolfia serpentina | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.3.3.2 | STR1 | - |
Rauvolfia serpentina |
4.3.3.2 | strictosidine synthase | - |
Rauvolfia serpentina |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.3.3.2 | 0.025 | - |
5-hydroxy-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 0.03 | 0.55 | 5-methyl-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 0.057 | 0.65 | tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 0.057 | 0.65 | 6-methyl-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 0.21 | - |
5-methoxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 0.52 | - |
6-fluoro-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 0.64 | - |
tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 0.97 | - |
6-methoxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 0.99 | - |
5-hydroxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 2.32 | - |
6-methyl-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 3.4 | - |
5-fluoro-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 3.8 | - |
5-fluoro-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 5.32 | - |
6-methoxy-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 6.56 | - |
5-methyl-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 10.65 | - |
tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 10.95 | - |
6-methyl-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 13.63 | - |
6-fluoro-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 16.66 | - |
6-methoxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 16.9 | - |
6-fluoro-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 18.12 | - |
5-hydroxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 21.24 | - |
5-fluoro-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 23.37 | - |
6-fluoro-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 37.46 | - |
5-fluoro-tryptamine | wild-type enzyme | Rauvolfia serpentina | |
4.3.3.2 | 54.09 | - |
tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 79.66 | - |
5-methoxy-tryptamine | mutant V208A | Rauvolfia serpentina | |
4.3.3.2 | 562.1 | - |
5-hydroxy-tryptamine | wild-type enzyme | Rauvolfia serpentina |