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Literature summary extracted from

  • Benoit, I.; Navarro, D.; Marnet, N.; Rakotomanomana, N.; Lesage-Meessen, L.; Sigoillot, J.C.; Asther, M.; Asther, M.
    Feruloyl esterases as a tool for the release of phenolic compounds from agro-industrial by-products (2006), Carbohydr. Res., 341, 1820-1827.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.73 degradation feruloyl esterase B is a tool for the release of phenolic compounds from agro-industrial by-products (coffe pulp, apple marc, wheat straw, sugar beet pulp and maite bran) Aspergillus niger
3.1.1.73 degradation feruloyl esterases A is a tool for the release of phenolic compounds from agro-industrial by-products (wheat straw, sugar beet pulp and maize bran) Aspergillus niger

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.73 0.02
-
 methyl p-coumarate isoenzyme FAEB Aspergillus niger
3.1.1.73 0.044
-
 O-[5-O-(trans-feruloyl)-alpha-L-Araf]-(1,3)D-Xylp isoenzyme FAEB Aspergillus niger
3.1.1.73 0.044
-
 coniferyl p-coumarate isoenzyme FAEB Aspergillus niger
3.1.1.73 0.045
-
 O-[5-O-(trans-feruloyl)-alpha-L-Araf]-(1,3)D-Xylp
-
 Aspergillus niger
3.1.1.73 0.061
-
 O-[5-O-(trans-feruloyl)-alpha-L-Araf]
-
 Aspergillus niger
3.1.1.73 0.103
-
 methyl sinapinate
-
 Aspergillus niger
3.1.1.73 0.132
-
 O-[5-O-(trans-feruloyl)-alpha-L-Araf] isoenzyme FAEB Aspergillus niger
3.1.1.73 0.158
-
 methyl caffeate isoenzyme FAEB Aspergillus niger
3.1.1.73 0.163
-
 methyl ferulate isoenzyme FAEB Aspergillus niger
3.1.1.73 0.163
-
 5-O-p-coumaroylquinic acid isoenzyme FAEB Aspergillus niger
3.1.1.73 0.237
-
 coniferyl p-coumarate
-
 Aspergillus niger
3.1.1.73 0.245
-
 chlorogenic acid isoenzyme FAEB Aspergillus niger
3.1.1.73 0.308
-
 methyl ferulate
-
 Aspergillus niger

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.73 Aspergillus niger O42807
-
-
3.1.1.73 Aspergillus niger Q8WZI8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.73 5-O-p-coumaroylquinic acid + H2O
-
 Aspergillus niger p-coumaric acid + quinic acid
-
 ?
3.1.1.73 chlorogenic acid + H2O
-
 Aspergillus niger ?
-
 ?
3.1.1.73 coniferyl p-coumarate + H2O
-
 Aspergillus niger p-coumaric acid + coniferol
-
 ?
3.1.1.73 methyl caffeate + H2O
-
 Aspergillus niger caffeic acid + methanol
-
 ?
3.1.1.73 methyl ferulate + H2O
-
 Aspergillus niger ferulic acid + methanol
-
 ?
3.1.1.73 methyl p-coumarate + H2O
-
 Aspergillus niger p-coumaric acid + methanol
-
 ?
3.1.1.73 methyl sinapinate + H2O
-
 Aspergillus niger sinapic acid + methanol
-
 ?
3.1.1.73 additional information FAEA hydrolyzes quinic esters and ester linkages between phenolic acids and lignin monomer Aspergillus niger ?
-
 ?
3.1.1.73 O-[5-O-(trans-feruloyl)-alpha-L-Araf] + H2O
-
 Aspergillus niger trans-ferulate + alpha-L-arabinofuranose
-
 ?
3.1.1.73 O-[5-O-(trans-feruloyl)-alpha-L-Araf]-(1,3)D-Xylp + H2O
-
 Aspergillus niger trans-ferulate + 3-O-alpha-L-arabinofuranosyl-D-xylopyranose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.73 FAEA
-
 Aspergillus niger
3.1.1.73 FAEB
-
 Aspergillus niger
3.1.1.73 feruloyl esterase A
-
 Aspergillus niger
3.1.1.73 feruloyl esterase B
-
 Aspergillus niger

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.1.73 2 8 O-[5-O-(trans-feruloyl)-alpha-L-Araf] isoenzyme FAEB Aspergillus niger
3.1.1.73 4
-
 methyl ferulate isoenzyme FAEB Aspergillus niger
3.1.1.73 8
-
 O-[5-O-(trans-feruloyl)-alpha-L-Araf]-(1,3)D-Xylp isoenzyme FAEB Aspergillus niger
3.1.1.73 16
-
 methyl ferulate isoenzyme FAEA Aspergillus niger
3.1.1.73 20
-
 coniferyl p-coumarate
-
 Aspergillus niger
3.1.1.73 48
-
 5-O-p-coumaroylquinic acid isoenzyme FAEB Aspergillus niger
3.1.1.73 61
-
 methyl sinapinate
-
 Aspergillus niger
3.1.1.73 63
-
 chlorogenic acid isoenzyme FAEB Aspergillus niger
3.1.1.73 69
-
 methyl p-coumarate isoenzyme FAEB Aspergillus niger
3.1.1.73 95
-
 O-[5-O-(trans-feruloyl)-alpha-L-Araf]-(1,3)D-Xylp
-
 Aspergillus niger
3.1.1.73 121
-
 O-[5-O-(trans-feruloyl)-alpha-L-Araf]
-
 Aspergillus niger
3.1.1.73 140
-
 coniferyl p-coumarate isoenzyme FAEB Aspergillus niger
3.1.1.73 234
-
 methyl caffeate isoenzyme FAEB Aspergillus niger