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Literature summary extracted from
- Inulin fructotransferase (DFA III-producing) from Leifsonia sp. T88-4 (2006), Carbohydr. Polym., 66, 75-80.
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.2.18 | 1 | - |
inulin | - |
Leifsonia sp. T88-4 |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 44000 | - |
2 * 44000, SDS-PAGE | Leifsonia sp. T88-4 |
| 4.2.2.18 | 74000 | - |
gel filtration | Leifsonia sp. T88-4 |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.18 | Leifsonia sp. T88-4 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.2.18 | - |
Leifsonia sp. T88-4 |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.2.2.18 | culture supernatant | - |
Leifsonia sp. T88-4 | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 644 | - |
di-D-fructofuranose 1,2':2,3' dianhydride produced | Leifsonia sp. T88-4 |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.18 | inulin | - |
Leifsonia sp. T88-4 | 3 di-D-fructofuranose 1,2':2,3' dianhydride + ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.2.18 | dimer | 2 * 44000, SDS-PAGE | Leifsonia sp. T88-4 |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 65 | - |
- |
Leifsonia sp. T88-4 |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 40 | 75 | 40°C: about 45% of maximal activity, 75°C: about 25% of maximal activity | Leifsonia sp. T88-4 |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 60 | - |
30 min, stable | Leifsonia sp. T88-4 |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 5 | - |
- |
Leifsonia sp. T88-4 |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.18 | 4 | 7 | pH 4.0: about 20% of maximal activity, pH 7.0: about 60% of maximal activity | Leifsonia sp. T88-4 |
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