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Literature summary extracted from

  • Bhatti, H.N.; Rashid, M.H.; Asgher, M.; Nawaz, R.; Khalid, A.M.; Perveen, R.
    Chemical modification results in hyperactivation and thermostabilization of Fusarium solani glucoamylase (2007), Can. J. Microbiol., 53, 177-185.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.3 EDTA chemical modification of the enzyme's carboxyl groups with EDTA as nucleophile in presence of 1-ethyl-3(3-dimethylaminopropyl)carbodiimide leads to a dramatic enhancement of enzyme catalytic activity and thermal stability Fusarium solani

General Stability

EC Number General Stability Organism
3.2.1.3 the EDTA-coupled enzyme is more stable towards proteases and urea Fusarium solani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.3 additional information
-
 additional information kinetics and thermodynamics of native and EDTA-coupled enzyme, overview Fusarium solani
3.2.1.3 1.6
-
 starch pH 5.5, 40°C, native enzyme Fusarium solani
3.2.1.3 2.3 2.9 starch pH 5.5, 40°C, EDTA-coupled enzyme Fusarium solani

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.3 starch + H2O Fusarium solani
-
 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Fusarium solani
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.3 native enzyme to homogeneity by anion exchange and hydrophobic interaction chromatography, and gel filtration Fusarium solani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.3 additional information solid-state fermentation on wheat bran Fusarium solani
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 additional information
-
-
 Fusarium solani

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 starch + H2O
-
 Fusarium solani ?
-
 ?
3.2.1.3 starch + H2O soluble starch Fusarium solani ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 glucoamylase
-
 Fusarium solani

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.3 40
-
 assay at Fusarium solani

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.3 20 75
-
 Fusarium solani

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.3 50
-
 half-life of native enzyme is 128 min Fusarium solani
3.2.1.3 60
-
 half-life of native enzyme is 25 min Fusarium solani

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.3 217
-
 starch pH 5.5, 40°C, native enzyme Fusarium solani
3.2.1.3 485 518 starch pH 5.5, 40°C, EDTA-coupled enzyme Fusarium solani

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4.5
-
 native enzyme Fusarium solani
3.2.1.3 5 6 EDTA-coupled enzyme Fusarium solani

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.3 2 9
-
 Fusarium solani