BRENDA - Enzyme Database

Inactivation of enzymes within spores of Bacillus megaterium ATCC 19213 by hydroperoxides

Palop, A.; Rutherford, G.C.; Marquis, R.E.; Can. J. Microbiol. 44, 465-470 (1998)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.49
cumene hydroperoxide
1% residual activity after treatment with 17 mM cumene hydroperoxide at 50°C and pH 7 for 2 h
Bacillus megaterium
1.1.1.49
hydrogen peroxide
inhibitory at 0.25%, at pH 7
Bacillus megaterium
1.1.1.49
peracetic acid
1% residual activity after treatment with 4 mM peracetic acid at 25°C and pH 7 for 15 min
Bacillus megaterium
1.1.1.49
tert-butyl hydroperoxide
1% residual activity after treatment with 290 mM tert-butyl hydroperoxide at 50°C and pH 7 for 3 h
Bacillus megaterium
2.7.1.40
cumene hydroperoxide
1% residual activity after treatment with 17 mM cumene hydroperoxide at 50°C and pH 7 for 2 h
Bacillus megaterium
2.7.1.40
hydrogen peroxide
inhibitory at 0.25%, at pH 7
Bacillus megaterium
2.7.1.40
peracetic acid
1% residual activity after treatment with 4 mM peracetic acid at 25°C and pH 7 for 15 min
Bacillus megaterium
2.7.1.40
tert-butyl hydroperoxide
1% residual activity after treatment with 290 mM tert-butyl hydroperoxide at 50°C and pH 7 for 3 h
Bacillus megaterium
4.1.2.13
hydrogen peroxide
inhibitory at 0.25%, at pH 7
Bacillus megaterium
4.2.1.11
cumene hydroperoxide
1% residual activity after treatment with 17 mM cumene hydroperoxide at 50°C and pH 7 for 2 h
Bacillus megaterium
4.2.1.11
hydrogen peroxide
inhibitory at 0.25%, at pH 7
Bacillus megaterium
4.2.1.11
peracetic acid
1% residual activity after treatment with 4 mM peracetic acid at 25°C and pH 7 for 15 min
Bacillus megaterium
4.2.1.11
tert-butyl hydroperoxide
1% residual activity after treatment with 290 mM tert-butyl hydroperoxide at 50°C and pH 7 for 3 h
Bacillus megaterium
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.49
Bacillus megaterium
-
strain ATCC 19213
-
2.7.1.40
Bacillus megaterium
-
strain ATCC 19213
-
4.1.2.13
Bacillus megaterium
-
strain ATCC 19213
-
4.2.1.11
Bacillus megaterium
-
strain ATCC 19213
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.49
D-glucose 6-phosphate + NADP+
-
678997
Bacillus megaterium
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
?
2.7.1.40
ATP + pyruvate
-
678997
Bacillus megaterium
ADP + phosphoenolpyruvate
-
-
-
?
4.1.2.13
D-fructose 1,6-bisphosphate
-
678997
Bacillus megaterium
glycerone phosphate + D-glyceraldehyde 3-phosphate
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
678997
Bacillus megaterium
phosphoenolpyruvate + H2O
-
-
-
?
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.49
80
-
very sensitive to moist heat at 80°C
Bacillus megaterium
2.7.1.40
80
-
sensitive to heat at 80°C
Bacillus megaterium
4.1.2.13
80
-
very sensitive to moist heat at 80°C
Bacillus megaterium
4.2.1.11
80
-
sensitive to moist heat at 80°C
Bacillus megaterium
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.49
NADP+
-
Bacillus megaterium
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.1.49
NADP+
-
Bacillus megaterium
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.49
cumene hydroperoxide
1% residual activity after treatment with 17 mM cumene hydroperoxide at 50°C and pH 7 for 2 h
Bacillus megaterium
1.1.1.49
hydrogen peroxide
inhibitory at 0.25%, at pH 7
Bacillus megaterium
1.1.1.49
peracetic acid
1% residual activity after treatment with 4 mM peracetic acid at 25°C and pH 7 for 15 min
Bacillus megaterium
1.1.1.49
tert-butyl hydroperoxide
1% residual activity after treatment with 290 mM tert-butyl hydroperoxide at 50°C and pH 7 for 3 h
Bacillus megaterium
2.7.1.40
cumene hydroperoxide
1% residual activity after treatment with 17 mM cumene hydroperoxide at 50°C and pH 7 for 2 h
Bacillus megaterium
2.7.1.40
hydrogen peroxide
inhibitory at 0.25%, at pH 7
Bacillus megaterium
2.7.1.40
peracetic acid
1% residual activity after treatment with 4 mM peracetic acid at 25°C and pH 7 for 15 min
Bacillus megaterium
2.7.1.40
tert-butyl hydroperoxide
1% residual activity after treatment with 290 mM tert-butyl hydroperoxide at 50°C and pH 7 for 3 h
Bacillus megaterium
4.1.2.13
hydrogen peroxide
inhibitory at 0.25%, at pH 7
Bacillus megaterium
4.2.1.11
cumene hydroperoxide
1% residual activity after treatment with 17 mM cumene hydroperoxide at 50°C and pH 7 for 2 h
Bacillus megaterium
4.2.1.11
hydrogen peroxide
inhibitory at 0.25%, at pH 7
Bacillus megaterium
4.2.1.11
peracetic acid
1% residual activity after treatment with 4 mM peracetic acid at 25°C and pH 7 for 15 min
Bacillus megaterium
4.2.1.11
tert-butyl hydroperoxide
1% residual activity after treatment with 290 mM tert-butyl hydroperoxide at 50°C and pH 7 for 3 h
Bacillus megaterium
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.49
D-glucose 6-phosphate + NADP+
-
678997
Bacillus megaterium
D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
-
-
?
2.7.1.40
ATP + pyruvate
-
678997
Bacillus megaterium
ADP + phosphoenolpyruvate
-
-
-
?
4.1.2.13
D-fructose 1,6-bisphosphate
-
678997
Bacillus megaterium
glycerone phosphate + D-glyceraldehyde 3-phosphate
-
-
-
?
4.2.1.11
2-phospho-D-glycerate
-
678997
Bacillus megaterium
phosphoenolpyruvate + H2O
-
-
-
?
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.1.1.49
80
-
very sensitive to moist heat at 80°C
Bacillus megaterium
2.7.1.40
80
-
sensitive to heat at 80°C
Bacillus megaterium
4.1.2.13
80
-
very sensitive to moist heat at 80°C
Bacillus megaterium
4.2.1.11
80
-
sensitive to moist heat at 80°C
Bacillus megaterium