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Literature summary extracted from

  • Lee, K.N.; Jackson, K.W.; Christiansen, V.J.; Lee, C.S.; Chun, J.; McKee, P.A.
    Antiplasmin-cleaving enzyme is a soluble form of fibroblast activation protein (2006), Blood, 107, 1397-1404.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.21.B28 FAP is expressed in Pichia pastoris Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.B28 0.026
-
 Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg pH 7.5, 22°C, APCE Homo sapiens
3.4.21.B28 0.029
-
 Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg pH 7.5, 22°C, recombinant FAP Homo sapiens
3.4.21.B28 0.101
-
 benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin pH 7.5, 22°C, recombinant FAP Homo sapiens
3.4.21.B28 0.124
-
 benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin pH 7.5, 22°C, APCE Homo sapiens
3.4.21.B28 0.272
-
 Ala-Pro-7-amido-4-trifluoromethylcoumarin pH 7.5, 22°C, recombinant FAP Homo sapiens
3.4.21.B28 0.323
-
 Ala-Pro-7-amido-4-trifluoromethylcoumarin pH 7.5, 22°C, APCE Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.B28 93000
-
 2 * 93000, recombinant FAP, SDS-PAGE Homo sapiens
3.4.21.B28 97000
-
 2 * 97000, native APCE, SDS-PAGE Homo sapiens
3.4.21.B28 153000
-
 recombinant FAP, gel filtration Homo sapiens
3.4.21.B28 175000
-
 native APCE, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.21.B28 Met-alpha2-antiplasmin + H2O Homo sapiens APCE and rFAP cleave both Pro3-Leu4 and Pro12-Asn13 bonds of Met-alpha2-antiplasmin, but relative kcat /Km values for Pro12-Asn13 are about 16fold higher than for Pro3-Leu4. Conversion of Met-alpha2-antiplasmin by membrane or soluble FAP to the more easily fibrin-incorporable form, Asn-alpha2-antiplasmin, may increase plasmin inhibition within fibrin surrounding certain neoplasms and have an impact on growth and therapeutic susceptibility ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.B28 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.B28
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.21.B28 blood plasma
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.B28 Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg + H2O
-
 Homo sapiens Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro + Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg
-
 ?
3.4.21.B28 benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin + H2O
-
 Homo sapiens benzyloxycarbonyl-Gly-Pro + 7-amino-4-methylcoumarin
-
 ?
3.4.21.B28 L-Ala-Pro-7-amido-4-trifluoromethylcoumarin + H2O
-
 Homo sapiens L-Ala-Pro + 7-amino-4-trifluoromethylcoumarin
-
 ?
3.4.21.B28 Met-alpha2-antiplasmin + H2O APCE and rFAP cleave both Pro3-Leu4 and Pro12-Asn13 bonds of Met-alpha2-antiplasmin, but relative kcat /Km values for Pro12-Asn13 are about 16fold higher than for Pro3-Leu4. Conversion of Met-alpha2-antiplasmin by membrane or soluble FAP to the more easily fibrin-incorporable form, Asn-alpha2-antiplasmin, may increase plasmin inhibition within fibrin surrounding certain neoplasms and have an impact on growth and therapeutic susceptibility Homo sapiens ?
-
 ?
3.4.21.B28 Met-alpha2-antiplasmin + H2O APCE and rFAP cleave both Pro3-Leu4 and Pro12-Asn13 bonds of Met-alpha2-antiplasmin, but relative kcat /Km values for Pro12-Asn13 are about 16fold higher than for Pro3-Leu4 Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.21.B28 dimer 2 * 93000, recombinant FAP, SDS-PAGE Homo sapiens
3.4.21.B28 dimer 2 * 97000, native APCE, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.21.B28 antiplasmin-cleaving enzyme soluble form of fibroblast activation protein Homo sapiens
3.4.21.B28 APCE soluble form of fibroblast activation protein Homo sapiens
3.4.21.B28 FAP
-
 Homo sapiens
3.4.21.B28 fibroblast activation protein
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.B28 0.51
-
 benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin pH 7.5, 22°C, recombinant FAP Homo sapiens
3.4.21.B28 0.54
-
 benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin pH 7.5, 22°C, APCE Homo sapiens
3.4.21.B28 0.995
-
 Ala-Pro-7-amido-4-trifluoromethylcoumarin APCE Homo sapiens
3.4.21.B28 1.06
-
 Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg pH 7.5, 22°C, APCE Homo sapiens
3.4.21.B28 1.08
-
 Ala-Pro-7-amido-4-trifluoromethylcoumarin pH 7.5, 22°C, recombinant FAP Homo sapiens
3.4.21.B28 1.2
-
 Arg-Lys(4-(4-dimethylaminophenylazo)benzoyl)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(5-[(2-aminoethyl)amino]-naphthalene-1-sulfonic acid)-Arg pH 7.5, 22°C, recombinant FAP Homo sapiens
3.4.21.B28 6.08
-
 Ala-Pro-7-amido-4-trifluoromethylcoumarin pH 7.5, 22°C, APCE Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.B28 7.5
-
 substrate: Arg-Lys(DABCYL)-Thr-Ser-Gly-Pro-Asn-Gln-Glu-Gln-Glu(EDANS)-Arg, APCE or rFAP Homo sapiens