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Literature summary extracted from

  • Lozada-Ramirez, J.D.; Martinez-Martinez, I.; Garcia-Carmona, F.; Sanchez-Ferrer, A.
    Cloning, overexpression, purification, and characterization of S-adenosylhomocysteine hydrolase from Corynebacterium efficiens YS-314 (2008), Biotechnol. Prog., 24, 120-127.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.13.2.1 expression in Escherichia coli, fusion protein Corynebacterium efficiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.13.2.1 0.0014
-
 S-adenosyl-DL-homocysteine 37°C Corynebacterium efficiens
3.13.2.1 0.01
-
 adenosine 37°C Corynebacterium efficiens
3.13.2.1 0.045
-
 DL-homocysteine 37°C Corynebacterium efficiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.13.2.1 52776
-
 4 * 52776, calculated Corynebacterium efficiens
3.13.2.1 210000
-
 gel filtration Corynebacterium efficiens

Organism

EC Number Organism UniProt Comment Textmining
3.13.2.1 Corynebacterium efficiens Q8FRJ4
-
-
3.13.2.1 Corynebacterium efficiens YS-314 Q8FRJ4
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.13.2.1 0.352
-
 37°C Corynebacterium efficiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.13.2.1 DL-homocysteine + adenosine
-
 Corynebacterium efficiens S-adenosyl-DL-homocysteine + H2O
-
 r
3.13.2.1 DL-homocysteine + adenosine
-
 Corynebacterium efficiens YS-314 S-adenosyl-DL-homocysteine + H2O
-
 r
3.13.2.1 S-adenosyl-DL-homocysteine + H2O
-
 Corynebacterium efficiens DL-homocysteine + adenosine
-
 r
3.13.2.1 S-adenosyl-DL-homocysteine + H2O
-
 Corynebacterium efficiens YS-314 DL-homocysteine + adenosine
-
 r

Subunits

EC Number Subunits Comment Organism
3.13.2.1 tetramer 4 * 52776, calculated Corynebacterium efficiens

Synonyms

EC Number Synonyms Comment Organism
3.13.2.1 SAHase
-
 Corynebacterium efficiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.13.2.1 40
-
-
 Corynebacterium efficiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.13.2.1 50
-
 10 min, almost complete loss of activity Corynebacterium efficiens