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Literature summary extracted from

  • Wu, Z.; Jiang, G.; Xiang, P.; Yang, D.; Wang, N.
    Purification and characterization of trypsin-like enzymes from North Pacific krill (Euphausia pacifica) (2008), Biotechnol. Lett., 30, 67-72.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.21.4 0.06
-
 N-alpha-benzoyl-L-arginine-p-nitroanilide isozyme TLE II, at 37°C, pH 8.0 Euphausia pacifica
3.4.21.4 0.09
-
 N-alpha-benzoyl-L-arginine-p-nitroanilide isozyme TLE I, at 37°C, pH 8.0 Euphausia pacifica

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.21.4 Ca2+ activates at concentrations above 0.4 mM Euphausia pacifica

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.21.4 32300
-
 TLE II, SDS-PAGE Euphausia pacifica
3.4.21.4 33000
-
 TLE I, SDS-PAGE Euphausia pacifica

Organism

EC Number Organism UniProt Comment Textmining
3.4.21.4 Euphausia pacifica
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.21.4 DEAE Sepharose FF column chromatography and Sephacryl S100 gel filtration Euphausia pacifica

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.21.4 0.52
-
 crude extract, at 37°C, pH 8.0 Euphausia pacifica
3.4.21.4 64
-
 isozyme TLE I after 123fold purification, at 37°C, pH 8.0 Euphausia pacifica
3.4.21.4 92
-
 isozyme TLE II after 177fold purification, at 37°C, pH 8.0 Euphausia pacifica

Storage Stability

EC Number Storage Stability Organism
3.4.21.4 25°C, crude extract, 30 days, retains more than 70% of the initial activity Euphausia pacifica
3.4.21.4 40°C, crude extract, 5-10 days, retains about 80% of the initial activity Euphausia pacifica
3.4.21.4 40°C, purified enzyme, 30 min, remains stable Euphausia pacifica

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.21.4 N-alpha-benzoyl-L-arginine-p-nitroanilide + H2O
-
 Euphausia pacifica N-alpha-benzoyl-L-arginine + p-nitroaniline
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.21.4 TLE I
-
 Euphausia pacifica
3.4.21.4 TLE II
-
 Euphausia pacifica
3.4.21.4 trypsin-like enzyme
-
 Euphausia pacifica

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.21.4 40 50
-
 Euphausia pacifica

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.21.4 10 70
-
 Euphausia pacifica

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.21.4 50
-
 activity is lost rapidly over 50°C Euphausia pacifica

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.21.4 0.84
-
 N-alpha-benzoyl-L-arginine-p-nitroanilide isozyme TLE I, at 37°C, pH 8.0 Euphausia pacifica
3.4.21.4 0.98
-
 N-alpha-benzoyl-L-arginine-p-nitroanilide isozyme TLE II, at 37°C, pH 8.0 Euphausia pacifica

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.21.4 9 10
-
 Euphausia pacifica

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.21.4 6 11
-
 Euphausia pacifica

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.4.21.4 Euphausia pacifica TLE II, isoelectric focusing
-
 4.3
3.4.21.4 Euphausia pacifica TLE I, isoelectric focusing
-
 4.5