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Literature summary extracted from

  • Kwon, T.; Kim, C.T.; Lee, J.H.
    Transglucosylation of ascorbic acid to ascorbic acid 2-glucoside by a recombinant sucrose phosphorylase from Bifidobacterium longum (2007), Biotechnol. Lett., 29, 611-615.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.7 synthesis enzymatic production of a chemically stable ascorbic acid derivative 2-O-alpha-D-glucopyranosyl-L-ascorbic acid Bifidobacterium longum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.7 Bifidobacterium longum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.7 recombinant enzyme partially by anion exchange chromatography Bifidobacterium longum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.7 4-nitophenyl-alpha-D-glucopyranoside + phosphate
-
 Bifidobacterium longum 4-nitrophenol + alpha-D-glucose 1-phosphate
-
 ?
2.4.1.7 sucrose + ascorbate
-
 Bifidobacterium longum D-fructose + 2-O-alpha-D-glucopyranosyl-L-ascorbic acid LC-MS product analysis ?
2.4.1.7 sucrose + L-ascorbic acid
-
 Bifidobacterium longum 2-O-alpha-D-glucopyranosyl-L-ascorbic acid + fructose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.7 SPase
-
 Bifidobacterium longum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.7 37
-
 assay at Bifidobacterium longum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.7 6.4 7.5 assay at, dependent on the substrate used Bifidobacterium longum