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Literature summary extracted from

  • Vijayakumar, G.R.; Divakar, S.
    Amyloglucosidase-catalyzed synthesis of eugenyl and curcuminyl glycosides (2007), Biotechnol. Lett., 29, 575-584.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.3 synthesis glycosylation of the phenolic hydroxyl group of the phenyl propanoid systems, eugenol and curcumin, using an amyloglucosidase from Rhizopus sp. and a beta-glucosidase from sweet almonds together with carbohydrates D-glucose, D-mannose, maltose, sucrose,and D-mannitol in di-isopropyl ether produce glycosides at 7-52% yields in 72 h, method optimization, overview, two compounds are glycosylated in order to enhance their water solubility and pharmacological activities Rhizopus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.3 Rhizopus sp.
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.3 commercial preparation
-
 Rhizopus sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.3 4.7
-
-
 Rhizopus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.3 curcumin + beta-D-glucose i.e. 1E,6E-1,7-di(4-hydroxy-3-methoxy-phenyl)-1,6-heptadiene-3,5-dione Rhizopus sp. curcuminyl bis-alpha-D-glucoside + 6-O-curcuminyl bis-D-glucose + curcuminyl bis-beta-D-glucoside
-
 ?
3.2.1.3 curcumin + D-mannitol i.e. 1E,6E-1,7-di(4-hydroxy-3-methoxy-phenyl)-1,6-heptadiene-3,5-dione Rhizopus sp. 1-O-curcuminyl bis-D-mannitol
-
 ?
3.2.1.3 curcumin + D-mannose i.e. 1E,6E-1,7-di(4-hydroxy-3-methoxy-phenyl)-1,6-heptadiene-3,5-dione Rhizopus sp. curcuminyl bis-alpha-D-mannoside + curcuminyl bis-maltoside + 6-O-curcuminyl bis-maltose + 6''-O-curcuminyl bis-maltose
-
 ?
3.2.1.3 curcumin + sucrose i.e. 1E,6E-1,7-di(4-hydroxy-3-methoxy-phenyl)-1,6-heptadiene-3,5-dione Rhizopus sp. 1-O-curcuminyl bis-sucrose + 6''-O-curcuminyl bis-sucrose + 6-O-curcuminyl bis-sucrose
-
 ?
3.2.1.3 eugenol + beta-D-glucose i.e. 4-allyl-2-methoxy phenol Rhizopus sp. eugenyl alpha-D-glucoside + 6-O-eugenyl D-glucose + eugenyl beta-D-glucoside
-
 ?
3.2.1.3 eugenol + D-mannitol i.e. 4-allyl-2-methoxy phenol Rhizopus sp. 1-O-eugenyl mannitol
-
 ?
3.2.1.3 eugenol + D-mannose i.e. 4-allyl-2-methoxy phenol Rhizopus sp. eugenyl alpha-D-mannoside
-
 ?
3.2.1.3 eugenol + maltose
-
 Rhizopus sp. eugenyl maltoside + 6-O-eugenyl maltose + 6''-O-eugenyl maltose
-
 ?
3.2.1.3 eugenol + sucrose i.e. 4-allyl-2-methoxy phenol Rhizopus sp. 1-O-eugenyl sucrose + 6-O-eugenyl sucrose + 6''-O-eugenyl sucrose
-
 ?
3.2.1.3 additional information reaction of the amyloglucosidase in concert with sweet almond beta-glucosidase, EC 3.2.1.21, to hydrolyze curcumin and eugenol in acetate buffer, method optimization, NMR product determinations, overview Rhizopus sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.3 amyloglucosidase
-
 Rhizopus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.3 4
-
 assay at Rhizopus sp.