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Literature summary extracted from

  • Voget, C.E.; Vita, C.E.; Contreras Esquivel, J.C.
    One-step concentration and partial purification of Aspergillus kawachii non-acidic polygalacturonases by adsorption to glass fiber microfilters (2006), Biotechnol. Lett., 28, 233-239.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.15 35500
-
 gel filtration, PG5 fraction PGIII Aspergillus luchuensis
3.2.1.15 38000
-
 gel filtration, PG5 fraction PGII Aspergillus luchuensis
3.2.1.15 41000
-
 41000, SDS-PAGE, PG5 fraction PGII Aspergillus luchuensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.15 Aspergillus luchuensis
-
-
-
3.2.1.15 Aspergillus luchuensis IFO 4308
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.15 one-step concentration and partial purification of PG5 by adsorption to glass fiber microfilters, 100fold. By gel filtration two PG5 fractions (PGII and PGII), 306fold Aspergillus luchuensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.15 15
-
 PG5 in fermentation broth Aspergillus luchuensis
3.2.1.15 1506
-
 100fold purified PG5 Aspergillus luchuensis
3.2.1.15 4585
-
 306fold purified PG5 fraction PGII Aspergillus luchuensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.15 polygalacturonic acid + H2O
-
 Aspergillus luchuensis ?
-
 ?
3.2.1.15 polygalacturonic acid + H2O
-
 Aspergillus luchuensis IFO 4308 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.15 monomer 41000, SDS-PAGE, PG5 fraction PGII Aspergillus luchuensis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.15 endo-PG
-
 Aspergillus luchuensis
3.2.1.15 non-acidic polygalacturonase
-
 Aspergillus luchuensis
3.2.1.15 PG5
-
 Aspergillus luchuensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.15 additional information
-
 optimum pHs for PG5 fractions PGII and PGIII towards polygalacturonic acid are 4.7 and 5, respectively Aspergillus luchuensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.15 additional information
-
 PG5 activity in the permeate decreases, as compared to fermentation broth, at pH values higher than 2.5 being negligible at pH 5.0 Aspergillus luchuensis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.15 Aspergillus luchuensis PG5 fraction PGII
-
 5.3