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Literature summary extracted from

  • Lee, D.G.; Park, G.T.; Kim, N.Y.; Lee, E.J.; Jang, M.K.; Shin, Y.G.; Park, G.S.; Kim, T.M.; Lee, J.H.; Lee, J.H.; Kim, S.J.; Lee, S.H.
    Cloning, expression, and characterization of a glycoside hydrolase family 50 beta-agarase from a marine Agarivorans isolate (2006), Biotechnol. Lett., 28, 1925-1932.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.81 expression in Escherichia coli Agarivorans sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.81 CaCl2 optimal activity in presence of 1 mM NaCl and 1 mM CaCl2 Agarivorans sp.
3.2.1.81 NaCl optimal activity in presence of 1 mM NaCl and 1 mM CaCl2 Agarivorans sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.81 109000
-
 x * 109450, calculated, x * 109000, SDS-PAGE Agarivorans sp.
3.2.1.81 109450
-
 x * 109450, calculated, x * 109000, SDS-PAGE Agarivorans sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.81 Agarivorans sp.
-
-
-
3.2.1.81 Agarivorans sp. JA-1
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.81 167
-
 pH 8.0, 40°C Agarivorans sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.81 agarose + H2O
-
 Agarivorans sp. neoagarosebiose main product ?
3.2.1.81 agarose + H2O
-
 Agarivorans sp. JA-1 neoagarosebiose main product ?
3.2.1.81 neoagarohexaose + H2O
-
 Agarivorans sp. neoagarobiose
-
 ?
3.2.1.81 neoagarohexaose + H2O
-
 Agarivorans sp. JA-1 neoagarobiose
-
 ?
3.2.1.81 neoagarotetraose + H2O
-
 Agarivorans sp. neoagarobiose
-
 ?
3.2.1.81 neoagarotetraose + H2O
-
 Agarivorans sp. JA-1 neoagarobiose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.81 ? x * 109450, calculated, x * 109000, SDS-PAGE Agarivorans sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.81 40
-
-
 Agarivorans sp.
3.2.1.81 60
-
 more than 70% of maximum activity Agarivorans sp.
3.2.1.81 70
-
 more than 50% of maximum activity Agarivorans sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.81 8
-
-
 Agarivorans sp.