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Literature summary extracted from

  • Ferrer, M.; Beloqui, A.; Golyshina, O.V.; Plou, F.J.; Neef, A.; Chernikova, T.N.; Fernandez-Arrojo, L.; Ghazi, I.; Ballesteros, A.; Elborough, K.; Timmis, K.N.; Golyshin, P.N.
    Biochemical and structural features of a novel cyclodextrinase from cow rumen metagenome (2007), Biotechnol. J., 2, 207-213.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.54 expression in Escherichia coli More

General Stability

EC Number General Stability Organism
3.2.1.54 stable in the presence of up to 2% Triton X-100, completely inactivated in the presence of SDS at concentrations higher than 50 mM More

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.54 EDTA
-
 More

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.54 Ca2+ slightly activating More
3.2.1.54 Mn2+ slightly activating More

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 280000
-
 SDS-PAGE, size exclusion column chromatography More

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 More
-
 DNA isolated from Bos taurus rumen fluid
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 of the recombinant protein More

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.54 80
-
 potato starch as substrate, maltose, kejibiose, nigeriose, iso-maltose, iso-maltotriose, trehalose and p-nitrophenyl ?-D-glucopyranoside are not used as substrates More
3.2.1.54 360
-
 amylopectin as substrate More
3.2.1.54 1640
-
 pullulan as substrate More
3.2.1.54 1680
-
 amylose n as substrate More
3.2.1.54 2600
-
 soluble starch as substrate More
3.2.1.54 3960
-
 cyclomaltooctaose as substrate More
3.2.1.54 4680
-
 cyclomaltohexaose as substrate More
3.2.1.54 4920
-
 cyclomaltoheptaose as substrate More
3.2.1.54 5000
-
 maltooligosaccharides as substrate More

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 amylopectin + H2O
-
 More D-glucose + ?
-
 ?
3.2.1.54 amylose + H2O
-
 More D-glucose + ?
-
 ?
3.2.1.54 cyclomaltodextrin + H2O
-
 More linear maltodextrin
-
 ?
3.2.1.54 cyclomaltoheptaose + H2O
-
 More D-glucose
-
 ?
3.2.1.54 cyclomaltohexaose + H2O
-
 More D-glucose
-
 ?
3.2.1.54 cyclomaltooctaose + H2O
-
 More D-glucose
-
 ?
3.2.1.54 maltooligosaccharides + H2O
-
 More D-glucose + ?
-
 ?
3.2.1.54 potato starch + H2O
-
 More D-glucose
-
 ?
3.2.1.54 pullulan + H2O
-
 More D-glucose + ?
-
 ?
3.2.1.54 soluble starch + H2O
-
 More D-glucose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 tetramer 4*70000 More

Synonyms

EC Number Synonyms Comment Organism
3.2.1.54 CDase
-
 More
3.2.1.54 cyclomaltodextrinase
-
 More
3.2.1.54 RA.04
-
 More

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 70
-
-
 More

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.54 60 80 half-life at 60°, 65°, 70° and 80°C is 600, 480, 30 and 12 min, respectively, independent on the pH and the presence of Ca2+ More

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.54 5.5 9
-
 More