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Literature summary extracted from

  • Lien, T.S.; Yu, S.T.; Wu, S.T.; Too, J.R.
    Induction and purification of a thermophilic chitinase produced by Aeromonas sp. DYU-Too7 using glucosamine (2007), Biotechnol. Bioprocess Eng., 12, 610-617.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.52 Ag+ 10 mM, 30% loss of activity Aeromonas sp.
3.2.1.52 Ba2+ 10 mM, 30% loss of activity Aeromonas sp.
3.2.1.52 Hg2+ 10 mM, 33% loss of activity Aeromonas sp.
3.2.1.52 Mg2+ 10 mM, 9% loss of activity Aeromonas sp.
3.2.1.52 Mn2+ 10 mM, 31% loss of activity Aeromonas sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.52 extracellular
-
 Aeromonas sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.52 36000
-
 x * 36000, SDS-PAGE Aeromonas sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.52 Aeromonas sp.
-
 DYU-Too7
-
3.2.1.52 Aeromonas sp. DYU-Too7
-
 DYU-Too7
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.52 recombinant enzyme Aeromonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.52 27.34
-
-
 Aeromonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.52 colloidal chitin + H2O exo-type chitinase Aeromonas sp. N,N'-diacetylchitobiose + ?
-
 ?
3.2.1.52 colloidal chitin + H2O exo-type chitinase Aeromonas sp. DYU-Too7 N,N'-diacetylchitobiose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.52 ? x * 36000, SDS-PAGE Aeromonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.52 50
-
-
 Aeromonas sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.52 10 70 10°C: about 50% of maximal activity, 70°C: about 45% of maximal activity Aeromonas sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.52 60
-
 pH 5.5, 1 h, stable below Aeromonas sp.
3.2.1.52 70
-
 pH 5.5, 1 h, 95% loss of activity Aeromonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.52 5
-
-
 Aeromonas sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.52 3 8 pH 3: about 15% of maximal activity, pH 8.0: about 50% of maximal activity Aeromonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.52 3 10 1 h, 40°C, enzyme retains almost 80% of its activity at pH 3.0 and at pH 10.0 Aeromonas sp.