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Literature summary extracted from

  • Yi, Y.; Kermasha, S.; Neufeld, R.
    Characterization of sol-gel entrapped chlorophyllase (2006), Biotechnol. Bioeng., 95, 840-849.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.14 acetone the activity of entrapped chlorophyllase increased with increasing acetone concentration from 6.5 to 20%, however, further increase of acetone leads to a decrease in chlorophyllase activity. Free chlorophyllase reaches its highest activity at acetone concentration of 10% in a medium consisting of water, acetone, and canola oil, overview Phaeodactylum tricornutum

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.14 additional information entrapping of the enzyme in tetramethoxysilane-based sol-gel in the presence of lipid, acetone is used to introduce the substrate-chlorophyll into aqueous reaction medium in solubilized form, the entrapped chlorophyllase is less sensitive toward the denaturing effect of acetone in the bulk phase than the free enzyme, sol-gel at wet-stage, diffusion coefficient and partition coefficient of chlorophyll in sol-gel, overview Phaeodactylum tricornutum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.14 canola oil presence of 10%, 20%, and 30% of canola oil decreased the activity of entrapped chlorophyllase by 60%, 75%, and 80%, respectively Phaeodactylum tricornutum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.14 additional information
-
additional information steady-state and non-steady-state kinetics of free enzyme and of enzyme trapped in a sol-gel medium, overview Phaeodactylum tricornutum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.14 chloroplast membrane
-
Phaeodactylum tricornutum 31969
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.14 chlorophyll + H2O Phaeodactylum tricornutum
-
phytol + chlorophyllide
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.14 Phaeodactylum tricornutum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.14 additional information
-
comparison of the catalytic performance of purified free enzyme and purified enzyme trapped in a sol-gel medium, overview Phaeodactylum tricornutum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.14 chlorophyll + H2O
-
Phaeodactylum tricornutum phytol + chlorophyllide
-
?
3.1.1.14 chlorophyll + H2O chlorophyll from Spinacia oleracea Phaeodactylum tricornutum phytol + chlorophyllide
-
?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.14 30
-
free enzyme and of enzyme trapped in a sol-gel medium Phaeodactylum tricornutum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.1.14 20 50
-
Phaeodactylum tricornutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.14 8
-
assay at Phaeodactylum tricornutum