EC Number | Cloned (Comment) | Organism |
---|---|---|
5.3.3.1 | expression of C-terminally His6-tagged enzyme as insoluble protein in inclusion bodies in Escherichia coli strain BLR(DE3) | Comamonas testosteroni |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.3.1 | 5-Androstene-3,17-dione | Comamonas testosteroni | - |
4-Androstene-3,17-dione | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
5.3.3.1 | Comamonas testosteroni | P00947 | i.e. Pseudomonas testosteroni | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.3.3.1 | recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BLR(DE3) to homogeneity by expanded bed adsorption column utilizing immobilized metal affinity chromatography, this integrated process greatly simplifies the recovery and purification of inclusion body proteins by removing the need for mechanical cell disruption, repeated inclusion body centrifugation, and difficult clarification operations, chemical extraction is as effective as homogenization at releasing the inclusion body proteins from the bacterial cells, over 99% purity of the enzyme, method optimization and validation, overview | Comamonas testosteroni |
EC Number | Renatured (Comment) | Organism |
---|---|---|
5.3.3.1 | dilution refolding of recombinant enzyme from inclusion bodies, denatured recombinant C-terminally His6-tagged enzyme 10fold with refolding buffer containing 50 mM potassium phosphate, pH 7.0, 4°C, incubation at 4°C for 12 h, urea 0.8 M | Comamonas testosteroni |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.3.3.1 | 5-Androstene-3,17-dione | - |
Comamonas testosteroni | 4-Androstene-3,17-dione | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
5.3.3.1 | DELTA5-3-ketosteroid isomerase | - |
Comamonas testosteroni |
5.3.3.1 | ketosteroid isomerase | - |
Comamonas testosteroni |
5.3.3.1 | KSI | - |
Comamonas testosteroni |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.3.3.1 | 30 | - |
assay at | Comamonas testosteroni |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.3.3.1 | 7 | - |
assay at | Comamonas testosteroni |