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Literature summary extracted from

  • Richter, S.; Nieveler, J.; Schulze, H.; Bachmann, T.T.; Schmid, R.D.
    High yield production of a mutant Nippostrongylus brasiliensis acetylcholinesterase in Pichia pastoris and its purification (2006), Biotechnol. Bioeng., 93, 1017-1022.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.1.1.7 diagnostics application in AChE biosensor Nippostrongylus brasiliensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.7 AChE B gene, high-yield overexpression of enzyme mutant M301A in Pichia pastoris strain X33 as secreted protein, high-cell-density fermentation, overview Nippostrongylus brasiliensis

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.7 M301A site-directed mutagenesis, mutation in the acyl pocket of the active site Nippostrongylus brasiliensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.7 chlorpyrifos strong inhibition, used for active site titration Nippostrongylus brasiliensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.7 69000
-
about, recombinant enzyme, gel filtration Nippostrongylus brasiliensis

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.7 Nippostrongylus brasiliensis
-
AChE B gene
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.7 recombinant enzyme mutant M301A 1.9fold from Pichia pastoris strain X33 culture supernatant by cross-flow filtration and anion-exchange chromatography to 97% purity/near homogeneity with a yield of 87%, large sclae purification method development Nippostrongylus brasiliensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.7 3300 3400 purified recombinant enzyme mutant M301A Nippostrongylus brasiliensis

Subunits

EC Number Subunits Comment Organism
3.1.1.7 monomer 1 * 69000, recombinant enzyme, SDS-PAGE Nippostrongylus brasiliensis

Synonyms

EC Number Synonyms Comment Organism
3.1.1.7 AChE
-
Nippostrongylus brasiliensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.7 7.2
-
assay at Nippostrongylus brasiliensis