Literature summary extracted from
Richter, S.; Nieveler, J.; Schulze, H.; Bachmann, T.T.; Schmid, R.D.
High yield production of a mutant Nippostrongylus brasiliensis acetylcholinesterase in Pichia pastoris and its purification (2006), Biotechnol. Bioeng., 93, 1017-1022.
Application
EC Number |
Application |
Comment |
Organism |
---|
3.1.1.7 |
diagnostics |
application in AChE biosensor |
Nippostrongylus brasiliensis |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.1.7 |
AChE B gene, high-yield overexpression of enzyme mutant M301A in Pichia pastoris strain X33 as secreted protein, high-cell-density fermentation, overview |
Nippostrongylus brasiliensis |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.1.1.7 |
M301A |
site-directed mutagenesis, mutation in the acyl pocket of the active site |
Nippostrongylus brasiliensis |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.1.7 |
chlorpyrifos |
strong inhibition, used for active site titration |
Nippostrongylus brasiliensis |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.1.7 |
69000 |
- |
about, recombinant enzyme, gel filtration |
Nippostrongylus brasiliensis |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.1.7 |
Nippostrongylus brasiliensis |
- |
AChE B gene |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.1.7 |
recombinant enzyme mutant M301A 1.9fold from Pichia pastoris strain X33 culture supernatant by cross-flow filtration and anion-exchange chromatography to 97% purity/near homogeneity with a yield of 87%, large sclae purification method development |
Nippostrongylus brasiliensis |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
3.1.1.7 |
3300 |
3400 |
purified recombinant enzyme mutant M301A |
Nippostrongylus brasiliensis |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.1.7 |
monomer |
1 * 69000, recombinant enzyme, SDS-PAGE |
Nippostrongylus brasiliensis |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.1.7 |
AChE |
- |
Nippostrongylus brasiliensis |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
3.1.1.7 |
7.2 |
- |
assay at |
Nippostrongylus brasiliensis |