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Literature summary extracted from

  • Horibata, Y.; Sueyoshi, N.; Ito, M.
    Expression of a functional sphingomyelinase of Pseudomonas sp. TK4 in mammalian cells (2007), Biosci. Biotechnol. Biochem., 71, 603-606.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 functional expression of a chimeric GFP-tagged SMase fused to a lysosomal sorting motif of lysosomal acid phosphatase in HEK-293 and CHO cells, sorting of the recombinant enzyme to lysosomes Pseudomonas sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Mn2+
-
 Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 sphingomyelin + H2O Pseudomonas sp.
-
 N-acylsphingosine + choline phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Pseudomonas sp.
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 sphingomyelin + H2O
-
 Pseudomonas sp. N-acylsphingosine + choline phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 SMase
-
 Pseudomonas sp.
3.1.4.12 sphingomyelinase
-
 Pseudomonas sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.12 37
-
 assay at Pseudomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 7.5
-
 assay at Pseudomonas sp.