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Literature summary extracted from

  • Dong, J.; Tamaru, Y.; Araki, T.
    Molecular cloning, expression, and characterization of a beta-agarase gene, agaD, from a marine bacterium, Vibrio sp. strain PO-303 (2007), Biosci. Biotechnol. Biochem., 71, 38-46.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.81 CaCl2 stimulation Vibrio sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.81 expression in Escherichia coli of full-length protein, of the catalytic module, and of the carbohydrate binding module Vibrio sp.

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.81 additional information expression in Escherichia coli of full-length protein, of the catalytic module, and of the carbohydrate binding module. The catalytic module alone exhibits 50fold higher acivity than the full-length enzyme Vibrio sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.81 AgNO3 1mM, complete inhibition Vibrio sp.
3.2.1.81 CuCl2 1mM, complete inhibition Vibrio sp.
3.2.1.81 CuSO4 1mM, complete inhibition Vibrio sp.
3.2.1.81 HgCl2 1mM, complete inhibition Vibrio sp.
3.2.1.81 Pb(CH3COO)2 1mM, complete inhibition Vibrio sp.
3.2.1.81 sodium dodecylsulfate 0.1%, complete inhibition Vibrio sp.
3.2.1.81 ZnCl2 1mM, complete inhibition Vibrio sp.
3.2.1.81 ZnSO4 1mM, complete inhibition Vibrio sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.81 additional information
-
additional information Km value of the catalytic module for agarose is 2.33 mg/ml Vibrio sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.81 50824
-
x * 50824, calculated Vibrio sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.81 Vibrio sp.
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.81 1.2
-
wild-type Vibrio sp.
3.2.1.81 63.6
-
catalytic module Vibrio sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.81 agarose + H2O
-
Vibrio sp. neoagarotetraose plus small amounts of neoagarohexaose and neoagarobiose ?
3.2.1.81 additional information no substrate: neoagarotetraose, kappa-carrageenan Vibrio sp. ?
-
?
3.2.1.81 neoagarohexaose + H2O
-
Vibrio sp. neoagarotetraose + neoagarobiose
-
?
3.2.1.81 porphyran + H2O
-
Vibrio sp. neoagaro-oligosaccharides
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.81 ? x * 50824, calculated Vibrio sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.81 AgaD
-
Vibrio sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.81 40
-
-
Vibrio sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.81 45
-
10 min, stable Vibrio sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.81 7.5
-
-
Vibrio sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.81 4 9 12 h, 25°C, retains more than 80% of activity Vibrio sp.