EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.4 | expression of mutant enzymes in Escherichia coli | Pyrococcus horikoshii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.1.4 | C159A | kcat/KM for p-nitrophenyl cellobiose is 1.8fold lower than wild-type value | Pyrococcus horikoshii |
3.2.1.4 | E163A | kcat/KM for p-nitrophenyl cellobiose is 6fold lower than wild-type value | Pyrococcus horikoshii |
3.2.1.4 | G158A | kcat/KM for p-nitrophenyl cellobiose is 2fold lower than wild-type value | Pyrococcus horikoshii |
3.2.1.4 | H155A | kcat/KM for p-nitrophenyl cellobiose is 140fold lower than wild-type value | Pyrococcus horikoshii |
3.2.1.4 | H161A | kcat/KM for p-nitrophenyl cellobiose is neatrly identical to wild-type value | Pyrococcus horikoshii |
3.2.1.4 | I157A | kcat/KM for p-nitrophenyl cellobiose is nearly identical to wild-type value | Pyrococcus horikoshii |
3.2.1.4 | I162A | kcat/KM for p-nitrophenyl cellobiose is 140fold lower than wild-type value | Pyrococcus horikoshii |
3.2.1.4 | P164A | kcat/KM for p-nitrophenyl cellobiose is 6fold lower than wild-type value | Pyrococcus horikoshii |
3.2.1.4 | R156A | kcat/KM for p-nitrophenyl cellobiose is 10fold lower than wild-type value | Pyrococcus horikoshii |
3.2.1.4 | T160A | kcat/KM for p-nitrophenyl cellobiose is nearly identical to wild-type value | Pyrococcus horikoshii |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.4 | 0.88 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme I157A | Pyrococcus horikoshii | |
3.2.1.4 | 0.92 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme T160A | Pyrococcus horikoshii | |
3.2.1.4 | 1.12 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, wild-type enzyme | Pyrococcus horikoshii | |
3.2.1.4 | 1.23 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme H161A | Pyrococcus horikoshii | |
3.2.1.4 | 1.37 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme G158A | Pyrococcus horikoshii | |
3.2.1.4 | 1.55 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme E163A | Pyrococcus horikoshii | |
3.2.1.4 | 1.58 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme P164A | Pyrococcus horikoshii | |
3.2.1.4 | 1.9 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme C159A | Pyrococcus horikoshii | |
3.2.1.4 | 4.57 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme I162A | Pyrococcus horikoshii | |
3.2.1.4 | 5.85 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme R156A | Pyrococcus horikoshii | |
3.2.1.4 | 6.69 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme H155A | Pyrococcus horikoshii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.4 | Pyrococcus horikoshii | O58925 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.4 | - |
Pyrococcus horikoshii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.4 | avicel + H2O | - |
Pyrococcus horikoshii | ? | - |
? | |
3.2.1.4 | carboxymethyl cellulose + H2O | - |
Pyrococcus horikoshii | ? | - |
? | |
3.2.1.4 | crystalline cellulase + H2O | - |
Pyrococcus horikoshii | ? | - |
? | |
3.2.1.4 | p-nitrophenyl cellobiose + H2O | - |
Pyrococcus horikoshii | p-nitrophenol + cellobiose | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.2.1.4 | 0.003 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme I162A | Pyrococcus horikoshii | |
3.2.1.4 | 0.006 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme H155A | Pyrococcus horikoshii | |
3.2.1.4 | 0.036 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme E163A | Pyrococcus horikoshii | |
3.2.1.4 | 0.037 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme P164A | Pyrococcus horikoshii | |
3.2.1.4 | 0.083 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme R156A | Pyrococcus horikoshii | |
3.2.1.4 | 0.092 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme G158A | Pyrococcus horikoshii | |
3.2.1.4 | 0.115 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme I157A | Pyrococcus horikoshii | |
3.2.1.4 | 0.129 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme T160A | Pyrococcus horikoshii | |
3.2.1.4 | 0.144 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme C159A | Pyrococcus horikoshii | |
3.2.1.4 | 0.157 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, wild-type enzyme | Pyrococcus horikoshii | |
3.2.1.4 | 0.167 | - |
p-nitrophenyl cellobiose | pH 5.5, 50°C, mutant enzyme H161A | Pyrococcus horikoshii |