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Literature summary extracted from

  • Kim, H.W.; Takagi, Y.; Hagihara, Y.; Ishikawa, K.
    Analysis of the putative substrate binding region of hyperthermophilic endoglucanase from Pyrococcus horikoshii (2007), Biosci. Biotechnol. Biochem., 71, 2585-2587.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression of mutant enzymes in Escherichia coli Pyrococcus horikoshii

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.4 C159A kcat/KM for p-nitrophenyl cellobiose is 1.8fold lower than wild-type value Pyrococcus horikoshii
3.2.1.4 E163A kcat/KM for p-nitrophenyl cellobiose is 6fold lower than wild-type value Pyrococcus horikoshii
3.2.1.4 G158A kcat/KM for p-nitrophenyl cellobiose is 2fold lower than wild-type value Pyrococcus horikoshii
3.2.1.4 H155A kcat/KM for p-nitrophenyl cellobiose is 140fold lower than wild-type value Pyrococcus horikoshii
3.2.1.4 H161A kcat/KM for p-nitrophenyl cellobiose is neatrly identical to wild-type value Pyrococcus horikoshii
3.2.1.4 I157A kcat/KM for p-nitrophenyl cellobiose is nearly identical to wild-type value Pyrococcus horikoshii
3.2.1.4 I162A kcat/KM for p-nitrophenyl cellobiose is 140fold lower than wild-type value Pyrococcus horikoshii
3.2.1.4 P164A kcat/KM for p-nitrophenyl cellobiose is 6fold lower than wild-type value Pyrococcus horikoshii
3.2.1.4 R156A kcat/KM for p-nitrophenyl cellobiose is 10fold lower than wild-type value Pyrococcus horikoshii
3.2.1.4 T160A kcat/KM for p-nitrophenyl cellobiose is nearly identical to wild-type value Pyrococcus horikoshii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 0.88
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme I157A Pyrococcus horikoshii
3.2.1.4 0.92
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme T160A Pyrococcus horikoshii
3.2.1.4 1.12
-
p-nitrophenyl cellobiose pH 5.5, 50°C, wild-type enzyme Pyrococcus horikoshii
3.2.1.4 1.23
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme H161A Pyrococcus horikoshii
3.2.1.4 1.37
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme G158A Pyrococcus horikoshii
3.2.1.4 1.55
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme E163A Pyrococcus horikoshii
3.2.1.4 1.58
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme P164A Pyrococcus horikoshii
3.2.1.4 1.9
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme C159A Pyrococcus horikoshii
3.2.1.4 4.57
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme I162A Pyrococcus horikoshii
3.2.1.4 5.85
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme R156A Pyrococcus horikoshii
3.2.1.4 6.69
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme H155A Pyrococcus horikoshii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Pyrococcus horikoshii O58925
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
Pyrococcus horikoshii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 avicel + H2O
-
Pyrococcus horikoshii ?
-
?
3.2.1.4 carboxymethyl cellulose + H2O
-
Pyrococcus horikoshii ?
-
?
3.2.1.4 crystalline cellulase + H2O
-
Pyrococcus horikoshii ?
-
?
3.2.1.4 p-nitrophenyl cellobiose + H2O
-
Pyrococcus horikoshii p-nitrophenol + cellobiose
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.4 0.003
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme I162A Pyrococcus horikoshii
3.2.1.4 0.006
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme H155A Pyrococcus horikoshii
3.2.1.4 0.036
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme E163A Pyrococcus horikoshii
3.2.1.4 0.037
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme P164A Pyrococcus horikoshii
3.2.1.4 0.083
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme R156A Pyrococcus horikoshii
3.2.1.4 0.092
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme G158A Pyrococcus horikoshii
3.2.1.4 0.115
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme I157A Pyrococcus horikoshii
3.2.1.4 0.129
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme T160A Pyrococcus horikoshii
3.2.1.4 0.144
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme C159A Pyrococcus horikoshii
3.2.1.4 0.157
-
p-nitrophenyl cellobiose pH 5.5, 50°C, wild-type enzyme Pyrococcus horikoshii
3.2.1.4 0.167
-
p-nitrophenyl cellobiose pH 5.5, 50°C, mutant enzyme H161A Pyrococcus horikoshii