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Literature summary extracted from

  • Murakami, S.; Nishimoto, H.; Toyama, Y.; Shimamoto, E.; Takenaka, S.; Kaulpiboon, J.; Prousoontorn, M.; Limpaseni, T.; Pongsawasdi, P.; Aoki, K.
    Purification and characterization of two alkaline, thermotolerant alpha-amylases from Bacillus halodurans 38C-2-1 and expression of the cloned gene in Escherichia coli (2007), Biosci. Biotechnol. Biochem., 71, 2393-2401.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1 expression in Escherichia coli, alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 expression in Escherichia coli, alpha-amylase II Halalkalibacterium halodurans

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 AgNO3 1 mM, 40°C, 30 min, 12% loss of activity, alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 BaCl2 1 mM, 40°C, 30 min, 16% loss of activity, alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 CoCl2 1 mM, 40°C, 30 min, 22% loss of activity, alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 CuSO4 1 mM, 40°C, 30 min, 25% loss of activity, alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 EDTA 1 mM, 40°C, 30 min, complete loss of activity, alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 FeCl3 1 mM, 40°C, 30 min, 21% loss of activity, alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 FeSO4 1 mM, 40°C, 30 min, 86% loss of activity, alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 HgCl2 1 mM, 40°C, 30 min, 95% loss of activity, alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 MnCl2 1 mM, 40°C, 30 min, 46% loss of activity, alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 NiSO4 1 mM, 40°C, 30 min, 79% loss of activity, alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 PbCl2 1 mM, 40°C, 30 min, 35% loss of activity, alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 ZnSO4 1 mM, 40°C, 30 min, 47% loss of activity, alpha-amylase I Halalkalibacterium halodurans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 10000
-
 alpha-amylase I, non-denaturing PAGE Halalkalibacterium halodurans
3.2.1.1 75000
-
 1 * 75000, alpha-amylase II, SDS-PAGE Halalkalibacterium halodurans
3.2.1.1 87000
-
 alpha-amylase II, non-denaturing PAGE Halalkalibacterium halodurans
3.2.1.1 100000
-
 1 * 100000, alpha-amylase I, SDS-PAGE Halalkalibacterium halodurans

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Halalkalibacterium halodurans
-
-
-
3.2.1.1 Halalkalibacterium halodurans 38C-2-1
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1 alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 alpha-amylase II Halalkalibacterium halodurans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 18
-
 alpha-amylase II Halalkalibacterium halodurans
3.2.1.1 130
-
 alpha-amylase I Halalkalibacterium halodurans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 amylopectin + H2O 121% of the activity with soluble starch Halalkalibacterium halodurans ?
-
 ?
3.2.1.1 amylopectin + H2O 121% of the activity with soluble starch Halalkalibacterium halodurans 38C-2-1 ?
-
 ?
3.2.1.1 amylose + H2O 30% of the activity with soluble starch Halalkalibacterium halodurans ?
-
 ?
3.2.1.1 amylose + H2O 30% of the activity with soluble starch Halalkalibacterium halodurans 38C-2-1 ?
-
 ?
3.2.1.1 glycogen + H2O 104% of the activity with soluble starch Halalkalibacterium halodurans ?
-
 ?
3.2.1.1 glycogen + H2O 104% of the activity with soluble starch Halalkalibacterium halodurans 38C-2-1 ?
-
 ?
3.2.1.1 additional information no hydrolytic activity towards pullulan or dextran Halalkalibacterium halodurans ?
-
 ?
3.2.1.1 additional information no hydrolytic activity towards pullulan or dextran Halalkalibacterium halodurans 38C-2-1 ?
-
 ?
3.2.1.1 soluble starch + H2O
-
 Halalkalibacterium halodurans ?
-
 ?
3.2.1.1 soluble starch + H2O
-
 Halalkalibacterium halodurans 38C-2-1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 monomer 1 * 100000, alpha-amylase I, SDS-PAGE Halalkalibacterium halodurans
3.2.1.1 monomer 1 * 75000, alpha-amylase II, SDS-PAGE Halalkalibacterium halodurans

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 alpha-amylase I
-
 Halalkalibacterium halodurans
3.2.1.1 alpha-amylase II
-
 Halalkalibacterium halodurans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 50
-
 alpha-amylase II Halalkalibacterium halodurans
3.2.1.1 50 60 alpha-amylase I Halalkalibacterium halodurans

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 30 60 30°C: 38% of maximal activity, 50-60: maximal activity, alpha-amylase II Halalkalibacterium halodurans
3.2.1.1 30 60 30°C: 42% of maximal activity, 50-60°C: maximal activity, alpha-amylase I Halalkalibacterium halodurans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 50
-
 30 min, 58% loss of activity, alpha-amylase II Halalkalibacterium halodurans
3.2.1.1 50
-
 30 min, less than 5% loss of activity, alpha-amylase I Halalkalibacterium halodurans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 10
-
 alpha-amylase I Halalkalibacterium halodurans
3.2.1.1 10 11 alpha-amylase I Halalkalibacterium halodurans

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 6 11 4°C, 24 h, stable, alpha-amylase I Halalkalibacterium halodurans