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Literature summary extracted from

  • Takashima, Y.; Senoura, T.; Yoshizaki, T.; Hamada, S.; Ito, H.; Matsui, H.
    Differential chain-length specificities of two isoamylase-type starch-debranching enzymes from developing seeds of kidney bean (2007), Biosci. Biotechnol. Biochem., 71, 2308-2312.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.68 60000
-
 1 * 60000, SDS-PAGE Phaseolus vulgaris
3.2.1.68 80000
-
 gel permeation chromatography Phaseolus vulgaris
3.2.1.68 87000
-
 heterotetramer with PvISA1, 2 * 87000 PyISA1 and 2 * 93000 PvISA2, SDS-PAGE Phaseolus vulgaris
3.2.1.68 87000
-
 heterotetramer with PvISA2, 2 * 87000 PvISA1 and 2 * 93000 PvISA2, SDS-PAGE Phaseolus vulgaris
3.2.1.68 93000
-
 heterotetramer with PvISA1, 2 * 87000 PyISA1 and 2 * 93000 PvISA2, SDS-PAGE Phaseolus vulgaris
3.2.1.68 93000
-
 heterotetramer with PvISA2, 2 * 87000 PvISA1 and 2 * 93000 PvISA2, SDS-PAGE Phaseolus vulgaris
3.2.1.68 370000
-
 gel permeation chromatography Phaseolus vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.68 Phaseolus vulgaris A4PIS8 kidney bean
-
3.2.1.68 Phaseolus vulgaris A4PIS9 kidney bean
-
3.2.1.68 Phaseolus vulgaris A4PIT0 kidney bean
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.68 Q sepharose column chromatography Phaseolus vulgaris

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.68 leaf
-
 Phaseolus vulgaris
-
3.2.1.68 seed
-
 Phaseolus vulgaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.68 0.77
-
 pullulan as substrate Phaseolus vulgaris
3.2.1.68 0.95
-
 pullulan as substrate Phaseolus vulgaris
3.2.1.68 3.51
-
 amylopectin as substrate Phaseolus vulgaris
3.2.1.68 6.58
-
 beta limit dextrin as substrate Phaseolus vulgaris
3.2.1.68 8.95
-
 gylcogen as substrate Phaseolus vulgaris
3.2.1.68 14.98
-
 amylopectin as substrate Phaseolus vulgaris
3.2.1.68 17.16
-
 gylcogen as substrate Phaseolus vulgaris
3.2.1.68 29.05
-
 beta limit dextrin as substrate Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.68 amylopectin + H2O
-
 Phaseolus vulgaris linear maltooligosaccharides
-
 ?
3.2.1.68 beta limit dextrin + H2O
-
 Phaseolus vulgaris linear maltooligosaccharides
-
 ?
3.2.1.68 glycogen + H2O
-
 Phaseolus vulgaris linear maltooligosaccharides
-
 ?
3.2.1.68 pullulan + H2O
-
 Phaseolus vulgaris linear maltooligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.68 monomer 1 * 60000, SDS-PAGE Phaseolus vulgaris
3.2.1.68 tetramer heterotetramer with PvISA1, 2 * 87000 PyISA1 and 2 * 93000 PvISA2, SDS-PAGE Phaseolus vulgaris
3.2.1.68 tetramer heterotetramer with PvISA2, 2 * 87000 PvISA1 and 2 * 93000 PvISA2, SDS-PAGE Phaseolus vulgaris

Synonyms

EC Number Synonyms Comment Organism
3.2.1.68 glycogen alpha-1,6-glucanohydrolase
-
 Phaseolus vulgaris
3.2.1.68 isoamylase
-
 Phaseolus vulgaris
3.2.1.68 PvISA1
-
 Phaseolus vulgaris
3.2.1.68 PvISA2
-
 Phaseolus vulgaris
3.2.1.68 PvISA3
-
 Phaseolus vulgaris