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Literature summary extracted from

  • Champreda, V.; Kanokratana, P.; Sriprang, R.; Tanapongpipat, S.; Eurwilaichitr, L.
    Purification, biochemical characterization, and gene cloning of a new extracellular thermotolerant and glucose tolerant maltooligosaccharide-forming alpha-amylase from an endophytic ascomycete Fusicoccum sp. BCC4124 (2007), Biosci. Biotechnol. Biochem., 71, 2010-2020.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.1 biotechnology the thermotolerant, glucose tolerant maltooligosaccharide-forming alpha-amylase is potent for biotechnological application Fusicoccum sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.1
-
 Fusicoccum sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Fe2+ 2 mM, complete inhibition Fusicoccum sp.
3.2.1.1 glucose 1 M, 34% inhibition. No inhibition up to 0.25 M Fusicoccum sp.
3.2.1.1 maltose 0.25 M, 69% inhibition Fusicoccum sp.
3.2.1.1 Mn2+ 2 mM, about 70% inhibition Fusicoccum sp.
3.2.1.1 Ni2+ 2 mM, about 15% inhibition Fusicoccum sp.
3.2.1.1 Zn2+ 2 mM, about 85% inhibition Fusicoccum sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.1 additional information
-
 additional information Km-value: 5.2 mg/ml for soluble starch, 14.46 mg/ml for beta-cyclodextrin, 9.18 mg/ml for gamma-cyclodextrin Fusicoccum sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.1 extracellular
-
 Fusicoccum sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Ca2+ 2 mM, about 1.2fold activation Fusicoccum sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 50000
-
 x * 50000, SDS-PAGE Fusicoccum sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Fusicoccum sp.
-
-
-
3.2.1.1 Fusicoccum sp. BCC4124
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1
-
 Fusicoccum sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.1 838.9
-
-
 Fusicoccum sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 beta-cyclodextrin + H2O
-
 Fusicoccum sp. ?
-
 ?
3.2.1.1 beta-cyclodextrin + H2O
-
 Fusicoccum sp. BCC4124 ?
-
 ?
3.2.1.1 gamma-cyclodextrin + H2O
-
 Fusicoccum sp. ?
-
 ?
3.2.1.1 gamma-cyclodextrin + H2O
-
 Fusicoccum sp. BCC4124 ?
-
 ?
3.2.1.1 maltoheptaose + H2O
-
 Fusicoccum sp. D-glucose + maltose + maltotriose
-
 ?
3.2.1.1 maltohexaose + H2O
-
 Fusicoccum sp. D-glucose + maltose + maltotriose
-
 ?
3.2.1.1 maltopentaose + H2O
-
 Fusicoccum sp. glucose + maltose + maltotriose
-
 ?
3.2.1.1 maltotetraose + H2O
-
 Fusicoccum sp. glucose + maltose + maltotriose
-
 ?
3.2.1.1 maltotriose + H2O
-
 Fusicoccum sp. maltose + D-glucose
-
 ?
3.2.1.1 maltotriose + H2O
-
 Fusicoccum sp. BCC4124 maltose + D-glucose
-
 ?
3.2.1.1 additional information trace activity towards alpha-cyclodextrin, no hydrolysis of pullulan, no hydrolysis of maltose Fusicoccum sp. ?
-
 ?
3.2.1.1 additional information trace activity towards alpha-cyclodextrin, no hydrolysis of pullulan, no hydrolysis of maltose Fusicoccum sp. BCC4124 ?
-
 ?
3.2.1.1 soluble starch + H2O maltose, maltotriose, and maltotetraose are the major products from starch hydrolysis but prolonged reaction leads to the production of glucose, maltose, and maltotriose Fusicoccum sp. ?
-
 ?
3.2.1.1 soluble starch + H2O maltose, maltotriose, and maltotetraose are the major products from starch hydrolysis but prolonged reaction leads to the production of glucose, maltose, and maltotriose Fusicoccum sp. BCC4124 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 ? x * 50000, SDS-PAGE Fusicoccum sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.1 Amy-FC1
-
 Fusicoccum sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 70
-
-
 Fusicoccum sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 30 75 30°C: about 60% of maximal activity, 75°C: about 85% of maximal activity Fusicoccum sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 50
-
 1 h, less than 5% loss of activity Fusicoccum sp.
3.2.1.1 60
-
 1 h, complete inactivation in absence of substrate, less than 10% inactivation in presence of soluble starch. In presence of Ca2+ and Sr2+, Amy-FC1 retains full activity after 1 h and more than 80% activity after 5 h Fusicoccum sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.1 0.025
-
 soluble starch
-
 Fusicoccum sp.
3.2.1.1 283.3
-
 beta-cyclodextrin
-
 Fusicoccum sp.
3.2.1.1 485
-
 beta-cyclodextrin
-
 Fusicoccum sp.
3.2.1.1 561.7
-
 soluble starch
-
 Fusicoccum sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 7
-
-
 Fusicoccum sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 5
-
 50°C, 1 h, about 40% loss of activity Fusicoccum sp.
3.2.1.1 7 8 50°C, 1 h, no loss of activity Fusicoccum sp.
3.2.1.1 9
-
 50°C, 1 h, about 60% loss of activity Fusicoccum sp.