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Literature summary extracted from

  • Matsumiya, M.; Arakane, Y.; Haga, A.; Muthukrishnan, S.; Kramer, K.J.
    Substrate specificity of chitinases from two species of fish, greenling, Hexagrammos otakii, and common mackerel, Scomber japonicus, and the insect, tobacco hornworm, Manduca sexta (2006), Biosci. Biotechnol. Biochem., 70, 971-979.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 additional information Km-value for glycolchitin is 0.182 mg/ml Scomber japonicus
3.2.1.14 additional information
-
 additional information Km-value for glycolchitin is 0.471 mg/ml, isoenzyme HoChiB Hexagrammos otakii
3.2.1.14 additional information
-
 additional information Km-value for glycolchitin is 0.494 mg/ml, isoenzyme HoChiC Hexagrammos otakii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 47000
-
 x * 47000, isoenzyme HoChiC, SDS-PAGE Hexagrammos otakii
3.2.1.14 51000
-
 x * 51000, isoenzyme HoChiB, SDS-PAGE Hexagrammos otakii
3.2.1.14 62000
-
 x * 62000, isoenzyme HoChiA, SDS-PAGE Hexagrammos otakii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Hexagrammos otakii
-
-
-
3.2.1.14 Manduca sexta
-
-
-
3.2.1.14 Scomber japonicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 isoenzyme HoChiA Hexagrammos otakii
3.2.1.14 isoenzyme HoChiB Hexagrammos otakii
3.2.1.14 isoenzyme HoChiC Hexagrammos otakii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 stomach isoenzyme HoChiA Hexagrammos otakii
-
3.2.1.14 stomach isoenzyme HoChiB Hexagrammos otakii
-
3.2.1.14 stomach isoenzyme HoChiC Hexagrammos otakii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 1.25
-
 isoenzyme HoChiB Hexagrammos otakii
3.2.1.14 1.25
-
 isoenzyme HoChiC Hexagrammos otakii
3.2.1.14 3.04
-
 isoenzyme HoChiA Hexagrammos otakii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O
-
 Scomber japonicus ?
-
 ?
3.2.1.14 chitin + H2O
-
 Manduca sexta ?
-
 ?
3.2.1.14 chitin + H2O alpha-chitin and beta-chitin, isoenzyme HoChiA Hexagrammos otakii ?
-
 ?
3.2.1.14 chitin + H2O alpha-chitin and beta-chitin, isoenzyme HoChiB Hexagrammos otakii ?
-
 ?
3.2.1.14 chitin + H2O alpha-chitin and beta-chitin, isoenzyme HoChiC Hexagrammos otakii ?
-
 ?
3.2.1.14 glycolchitin + H2O
-
 Scomber japonicus ?
-
 ?
3.2.1.14 glycolchitin + H2O isoenzyme HoChiA Hexagrammos otakii ?
-
 ?
3.2.1.14 glycolchitin + H2O isoenzyme HoChiB Hexagrammos otakii ?
-
 ?
3.2.1.14 glycolchitin + H2O isoenzyme HoChiC Hexagrammos otakii ?
-
 ?
3.2.1.14 additional information no activity with p-nitrophenyl-(GlcNAc) Scomber japonicus ?
-
 ?
3.2.1.14 additional information no activity with p-nitrophenyl-(GlcNAc), enzyme form MsChi386 Manduca sexta ?
-
 ?
3.2.1.14 additional information no activity with p-nitrophenyl-(GlcNAc), enzyme form MsChi535 Manduca sexta ?
-
 ?
3.2.1.14 additional information no activity with p-nitrophenyl-(GlcNAc), isoenzyme HoChiA Hexagrammos otakii ?
-
 ?
3.2.1.14 additional information no activity with p-nitrophenyl-(GlcNAc), isoenzyme HoChiB Hexagrammos otakii ?
-
 ?
3.2.1.14 additional information no activity with p-nitrophenyl-(GlcNAc), isoenzyme HoChiC Hexagrammos otakii ?
-
 ?
3.2.1.14 p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O 13% of the activity with p-nitrophenyl-N,N'-diacetylchitobiose, enzyme form MsChi535 Manduca sexta ?
-
 ?
3.2.1.14 p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O 27% of the activity with p-nitrophenyl-N,N'-diacetylchitobiose, enzyme form MsChi386 Manduca sexta ?
-
 ?
3.2.1.14 p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O 30% of the activity with p-nitrophenyl-N,N'-diacetylchitobiose Scomber japonicus ?
-
 ?
3.2.1.14 p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O isoenzyme HoChiA Hexagrammos otakii ?
-
 ?
3.2.1.14 p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O low activity, isoenzyme HoChiB Hexagrammos otakii ?
-
 ?
3.2.1.14 p-nitrophenyl beta-D-N',N'',N'''-triacetylchitotriose + H2O low activity, isoenzyme HoChiC Hexagrammos otakii ?
-
 ?
3.2.1.14 p-nitrophenyl-N,N'-diacetylchitobiose + H2O
-
 Scomber japonicus ?
-
 ?
3.2.1.14 p-nitrophenyl-N,N'-diacetylchitobiose + H2O enzyme form MsChi386 Manduca sexta ?
-
 ?
3.2.1.14 p-nitrophenyl-N,N'-diacetylchitobiose + H2O enzyme form MsChi535 Manduca sexta ?
-
 ?
3.2.1.14 p-nitrophenyl-N,N'-diacetylchitobiose + H2O isoenzyme HoChiA Hexagrammos otakii ?
-
 ?
3.2.1.14 p-nitrophenyl-N,N'-diacetylchitobiose + H2O isoenzyme HoChiB Hexagrammos otakii ?
-
 ?
3.2.1.14 p-nitrophenyl-N,N'-diacetylchitobiose + H2O isoenzyme HoChiC Hexagrammos otakii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 47000, isoenzyme HoChiC, SDS-PAGE Hexagrammos otakii
3.2.1.14 ? x * 51000, isoenzyme HoChiB, SDS-PAGE Hexagrammos otakii
3.2.1.14 ? x * 62000, isoenzyme HoChiA, SDS-PAGE Hexagrammos otakii

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 HoChiA
-
 Hexagrammos otakii
3.2.1.14 HoChiB
-
 Hexagrammos otakii
3.2.1.14 HoChiC
-
 Hexagrammos otakii
3.2.1.14 MsChi386
-
 Manduca sexta
3.2.1.14 MsChi535
-
 Manduca sexta
3.2.1.14 SjChi
-
 Scomber japonicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 60
-
 isoenzyme HoChiA, substrate: glycolchitin Hexagrammos otakii
3.2.1.14 70
-
 isoenzyme HoChiB, substrate: glycolchitin Hexagrammos otakii
3.2.1.14 70
-
 isoenzyme HoChiC, substrate: glycolchitin Hexagrammos otakii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 40 70 40°C: about 30% of maximal activity, 70°C: about 60% of maximal activity, isoenzyme HoChiA, substrate: glycolchitin Hexagrammos otakii
3.2.1.14 50 80 50°C: about 40% of maximal activity, 80°C: about 60% of maximal activity, isoenzyme HoChiB, substrate: glycolchitin Hexagrammos otakii
3.2.1.14 50 80 50°C: about 40% of maximal activity, 80°C: about 70% of maximal activity, isoenzyme HoChiC, substrate: glycolchitin Hexagrammos otakii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 1.06
-
 glycolchitin isoenzyme HoChiB Hexagrammos otakii
3.2.1.14 1.85
-
 glycolchitin isoenzyme HoChiC Hexagrammos otakii
3.2.1.14 2.21
-
 glycolchitin isoenzyme HoChiC Scomber japonicus
3.2.1.14 3.15
-
 glycolchitin isoenzyme HoChiA Hexagrammos otakii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 2
-
 the enzyme has highest activity around pH 2 and exhibits a second optimum at pH 8 with 35% of the maximal activity at pH 2, isoenzyme HoChiA Hexagrammos otakii
3.2.1.14 8
-
 isoenzyme HoChiB Hexagrammos otakii
3.2.1.14 8
-
 isoenzyme HoChiC Hexagrammos otakii
3.2.1.14 8
-
 the enzyme has highest activity around pH 2 and exhibits a second optimum at pH 8 with 35% of the maximal activity at pH 2, isoenzyme HoChiA Hexagrammos otakii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 1 8 pH 1.0: about 70% of maximal activity, pH 6.0: 15% of maximal activity, pH 8.0: about 35% of maximal activity Hexagrammos otakii
3.2.1.14 1 9 HoChiB has more than 50% activity from pH 1 to 9, and the highest activity is at pH 8 Hexagrammos otakii
3.2.1.14 1 9 HoChiC has more than 50% activity from pH 1 to 9, and the highest activity was at pH 8 Hexagrammos otakii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Hexagrammos otakii isoelectric focusing, pH range of 3.5-9.5, isoenzyme HoChiA
-
 5.7
3.2.1.14 Hexagrammos otakii isoelectric focusing, pH range of 3.5-9.5, isoenzyme HoChiB
-
 7.6
3.2.1.14 Hexagrammos otakii isoelectric focusing, pH range of 3.5–9.5, isoenzyme HoChiC
-
 8.8