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Literature summary extracted from

  • Ichinose, H.; Kotake, T.; Tsumuraya, Y.; Kaneko, S.
    Characterization of an exo-beta -1,3-D-galactanase from Streptomyces avermitilis NBRC14893 acting on arabinogalactan-proteins (2006), Biosci. Biotechnol. Biochem., 70, 2745-2750.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.145 expressed in Escherichia coli Streptomyces avermitilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.145 38000
-
 SDS-PAGE Streptomyces avermitilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.145 Streptomyces avermitilis
-
 NBCR14893
-
3.2.1.145 Streptomyces avermitilis NBRC 14893
-
 NBCR14893
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.145 additional information sequence 1-20 at the N-terminus is predicted to be a signal sequence Streptomyces avermitilis

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.145 Ni-NTA agarose column Streptomyces avermitilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.145 additional information
-
 3.7 units/mg, beta-1,3-galactan as a substrate Streptomyces avermitilis
3.2.1.145 additional information
-
 substrate beta-(1-3)-D-galactan: relative activity 100% Streptomyces avermitilis
3.2.1.145 additional information
-
 substrate beta-(1-3)-D-galactobiose: relative activity 7.6% Streptomyces avermitilis
3.2.1.145 additional information
-
 substrate beta-(1-3)-D-galactotriose: relative activity 57% Streptomyces avermitilis
3.2.1.145 additional information
-
 substrate methyl-beta-1,3-D-galactopentaoside: relative activity 100% Streptomyces avermitilis
3.2.1.145 additional information
-
 substrate methyl-beta-1,3-D-galactotetraoside: relative activity 99% Streptomyces avermitilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.145 beta-(1-3)-D-galactan + H2O relative activity: 100%. The enzyme hydrolizes only beta-1,3-linkage of two D-galactosyl residues at non-reducing ends of the substrate Streptomyces avermitilis beta-(1-3)-D-galactan + D-galactose
-
 ?
3.2.1.145 beta-(1-3)-D-galactan + H2O relative activity: 100%. The enzyme hydrolizes only beta-1,3-linkage of two D-galactosyl residues at non-reducing ends of the substrate Streptomyces avermitilis NBRC 14893 beta-(1-3)-D-galactan + D-galactose
-
 ?
3.2.1.145 beta-(1-3)-D-galactotriose + H2O relative activity: 57% Streptomyces avermitilis beta-(1-3)-D-galactobiose + D-galactose
-
 ?
3.2.1.145 beta-(1-3)-D-galactotriose + H2O relative activity: 57% Streptomyces avermitilis NBRC 14893 beta-(1-3)-D-galactobiose + D-galactose
-
 ?
3.2.1.145 beta-(1-3)-galactobiose + H2O relative activity: 7.6% Streptomyces avermitilis D-galactose
-
 ?
3.2.1.145 beta-(1-3)-galactobiose + H2O relative activity: 7.6% Streptomyces avermitilis NBRC 14893 D-galactose
-
 ?
3.2.1.145 methyl-beta-1,3-D-galactopentaoside + H2O relative activity: 100%. The enzyme hydolyzes beta-1,3-linked galactooligosaccharides, but not beta-1,6- and beta-1,4-linked galactooligosaccharides or alpha-1,3-linked galactobiose or beta-1,3-linked galactosylgalactosaminide Streptomyces avermitilis methyl-beta-1,3-D-galactotetraoside + D-galactose
-
 ?
3.2.1.145 methyl-beta-1,3-D-galactopentaoside + H2O relative activity: 100%. The enzyme hydolyzes beta-1,3-linked galactooligosaccharides, but not beta-1,6- and beta-1,4-linked galactooligosaccharides or alpha-1,3-linked galactobiose or beta-1,3-linked galactosylgalactosaminide Streptomyces avermitilis NBRC 14893 methyl-beta-1,3-D-galactotetraoside + D-galactose
-
 ?
3.2.1.145 methyl-beta-1,3-D-galactotetraoside + H2O relative activity: 99% Streptomyces avermitilis methyl-beta-1,3-D-galactotrioside + D-galactose
-
 ?
3.2.1.145 methyl-beta-1,3-D-galactotetraoside + H2O relative activity: 99% Streptomyces avermitilis NBRC 14893 methyl-beta-1,3-D-galactotrioside + D-galactose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.145 exo-beta-1,3-D -galactanase
-
 Streptomyces avermitilis
3.2.1.145 Sa1,3Gal43A
-
 Streptomyces avermitilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.145 37
-
-
 Streptomyces avermitilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.145 5
-
-
 Streptomyces avermitilis