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Literature summary extracted from

  • Ogura, J.; Toyoda, A.; Kurosawa, T.; Chong, A.L.; Chohnan, S.; Masaki, T.
    Purification, characterization, and gene analysis of cellulase (Cel8A) from Lysobacter sp. IB-9374 (2006), Biosci. Biotechnol. Biochem., 70, 2420-2428.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.4 EDTA 1 mM, 1.2fold activation Lysobacter sp.
3.2.1.4 NEM 1 mM, 1.4fold activation Lysobacter sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.4 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide 100 mM, 98% inhibition Lysobacter sp.
3.2.1.4 Hg2+ 1 mM, complete inhibition Lysobacter sp.
3.2.1.4 Monoiodoacetic acid 1 mM, 10% inhibition Lysobacter sp.
3.2.1.4 N-bromosuccinimide 1 mM, 99% inhibition Lysobacter sp.
3.2.1.4 o-phenanthroline 1 mM, 75% inhibition Lysobacter sp.
3.2.1.4 p-chloromercuribenzoate 0.01 mM, 20% inhibition Lysobacter sp.
3.2.1.4 PMSF 1 mM, 89% inhibition Lysobacter sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.132 extracellular
-
 Lysobacter sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 Ba2+ 1 mM, 1.3fold activation Lysobacter sp.
3.2.1.4 Ca2+ 1 mM, 1.3fold activation Lysobacter sp.
3.2.1.4 Mg2+ 1 mM, 1.3fold activation Lysobacter sp.
3.2.1.4 Zn2+ 1 mM, 1.3fold activation Lysobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 41000
-
 x * 41000, SDS-PAGE Lysobacter sp.
3.2.1.4 41241
-
 x * 41241, calculated from sequuence Lysobacter sp.
3.2.1.132 41000
-
 x * 41000, SDS-PAGE, x * 41241, calculated Lysobacter sp.
3.2.1.132 41241
-
 x * 41000, SDS-PAGE, x * 41241, calculated Lysobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Lysobacter sp. Q25C15
-
-
3.2.1.4 Lysobacter sp. IB-9374 Q25C15
-
-
3.2.1.132 Lysobacter sp.
-
 isoform Cel8A exhibiting both chitosanase and beta-1,4-glucanase activities
-
3.2.1.132 Lysobacter sp. IB-9374
-
 isoform Cel8A exhibiting both chitosanase and beta-1,4-glucanase activities
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.132 proteolytic modification sequence contains a 33-amino acid, N-terminal signal peptide and a mature enzyme Lysobacter sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Lysobacter sp.
3.2.1.132
-
 Lysobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 culture filtrate
-
 Lysobacter sp.
-
3.2.1.132 culture fluid
-
 Lysobacter sp.
-

Storage Stability

EC Number Storage Stability Organism
3.2.1.4 4°C, pH 6.5, 24 h , stable Lysobacter sp.
3.2.1.132 4°C, pH 6.5, stable for at least 24 h Lysobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O
-
 Lysobacter sp. cellobiose + ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Lysobacter sp. IB-9374 cellobiose + ?
-
 ?
3.2.1.4 cellohexaose + H2O major product is cellotriose, minor products are cellobiose and cellotetraose Lysobacter sp. cellotriose + cellotetraose + cellobiose
-
 ?
3.2.1.4 cellohexaose + H2O major product is cellotriose, minor products are cellobiose and cellotetraose Lysobacter sp. IB-9374 cellotriose + cellotetraose + cellobiose
-
 ?
3.2.1.4 additional information the enzyme also shows chitosanase activity. Colloidal chitosan, chitosan and glycol chitosan are hydrolyzed by Cel8A at 15-40% the activity of cellulose Lysobacter sp. ?
-
 ?
3.2.1.4 additional information the enzyme also shows chitosanase activity. Colloidal chitosan, chitosan and glycol chitosan are hydrolyzed by Cel8A at 15-40% the activity of cellulose Lysobacter sp. IB-9374 ?
-
 ?
3.2.1.132 chitosan + H2O substrate chitosan, 99% deacetylated, 67% of the activity with colloidal chitosan Lysobacter sp. chitosan oligosaccharides
-
 ?
3.2.1.132 chitosan + H2O substrate colloidal chitosan, 85% deacetylated Lysobacter sp. chitosan oligosaccharides
-
 ?
3.2.1.132 chitosan + H2O substrate chitosan, 99% deacetylated, 67% of the activity with colloidal chitosan Lysobacter sp. IB-9374 chitosan oligosaccharides
-
 ?
3.2.1.132 chitosan + H2O substrate colloidal chitosan, 85% deacetylated Lysobacter sp. IB-9374 chitosan oligosaccharides
-
 ?
3.2.1.132 colloidal chitin + H2O 5.1% of the activity with colloidal chitosan Lysobacter sp. ?
-
 ?
3.2.1.132 colloidal chitin + H2O 5.1% of the activity with colloidal chitosan Lysobacter sp. IB-9374 ?
-
 ?
3.2.1.132 glycol chitosan + H2O substrate approximately 40% deacetylated, 38% of the activity with colloidal chitosan Lysobacter sp. glycol chitosan oligosaccharides
-
 ?
3.2.1.132 glycol chitosan + H2O substrate approximately 40% deacetylated, 38% of the activity with colloidal chitosan Lysobacter sp. IB-9374 glycol chitosan oligosaccharides
-
 ?
3.2.1.132 additional information at 37°C and pH 5.0, beta-1,4-glucanase activitiy is about 2.5fold higher than activity on colloidal chitosan Lysobacter sp. ?
-
 ?
3.2.1.132 additional information at 37°C and pH 5.0, beta-1,4-glucanase activitiy is about 2.5fold higher than activity on colloidal chitosan Lysobacter sp. IB-9374 ?
-
 ?
3.2.1.132 water-soluble chitosan + H2O substrate 40-50% deacetylated, 7.6% of the activity with colloidal chitosan Lysobacter sp. chitosan oligosaccharides
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 41000, SDS-PAGE Lysobacter sp.
3.2.1.4 ? x * 41241, calculated from sequuence Lysobacter sp.
3.2.1.132 ? x * 41000, SDS-PAGE, x * 41241, calculated Lysobacter sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 Cel8A
-
 Lysobacter sp.
3.2.1.132 Cel8A
-
 Lysobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 40
-
-
 Lysobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 20 60 20°C: about 55% of maximal activity, 60°C: about 60% of maximal activity Lysobacter sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 40
-
 stable up to Lysobacter sp.
3.2.1.132 40
-
 30 min, 20 mM acetate pH 5.0, stable up to 40°C Lysobacter sp.
3.2.1.132 50
-
 30 min, 20 mM acetate pH 5.0, 95% loss of activity Lysobacter sp.
3.2.1.132 70
-
 optimum for chitosanase acivity Lysobacter sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 5
-
 cellulase activity Lysobacter sp.
3.2.1.132 5
-
 and 7.0, at pH 7.0 higher chitosanase activity than at pH 5.0 Lysobacter sp.
3.2.1.132 7
-
 and 5.0, at pH 7.0 higher chitosanase activity than at pH 5.0 Lysobacter sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 4.5 6.5 pH 4.5: about 85% of maximal activity, pH 6.5: about 40% of maximal activity Lysobacter sp.
3.2.1.132 5 8 more than 80% of maximum activity Lysobacter sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 5 8 24 h, 4°C, more than 80% of original activity is retained Lysobacter sp.
3.2.1.4 6.5
-
 24 h, 4°C, most stable at Lysobacter sp.