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Literature summary extracted from

  • Tasaki, Y.; Yoshikawa, H.; Tamura, H.
    Isolation and characterization of an alcohol dehydrogenase gene from the octylphenol polyethoxylate degrader Pseudomonas putida S-5 (2006), Biosci. Biotechnol. Biochem., 70, 1855-1863.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.1 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.1.1 cytoplasm 68% of total activity measured Pseudomonas putida 5737
-
1.1.1.1 membrane 32% of total activity measured Pseudomonas putida 16020
-
1.1.1.1 additional information no activity can be localized in the periplasm Pseudomonas putida
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.1 56700
-
 deduced from amino acid sequence Pseudomonas putida
1.1.1.1 57000
-
 SDS-PAGE Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.1 Pseudomonas putida Q76HN6 probable alcohol dehydrogenase
-
1.1.1.1 Pseudomonas putida S-5 Q76HN6 probable alcohol dehydrogenase
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.1 1-butanol + NAD+ 30% activity compared to benzyl alcohol Pseudomonas putida butanal + NADH + H+
-
 ?
1.1.1.1 1-butanol + NAD+ 30% activity compared to benzyl alcohol Pseudomonas putida S-5 butanal + NADH + H+
-
 ?
1.1.1.1 1-decanol + NAD+ 13% activity compared to benzyl alcohol Pseudomonas putida decanal + NADH + H+
-
 ?
1.1.1.1 1-decanol + NAD+ 13% activity compared to benzyl alcohol Pseudomonas putida S-5 decanal + NADH + H+
-
 ?
1.1.1.1 1-heptanol + NAD+ 25% activity compared to benzyl alcohol Pseudomonas putida heptanal + NADH + H+
-
 ?
1.1.1.1 1-heptanol + NAD+ 25% activity compared to benzyl alcohol Pseudomonas putida S-5 heptanal + NADH + H+
-
 ?
1.1.1.1 1-hexanol + NAD+ 61% activity compared to benzyl alcohol Pseudomonas putida hexanal + NADH + H+
-
 ?
1.1.1.1 1-hexanol + NAD+ 61% activity compared to benzyl alcohol Pseudomonas putida S-5 hexanal + NADH + H+
-
 ?
1.1.1.1 1-nonanol + NAD+ 12% activity compared to benzyl alcohol Pseudomonas putida nonanal + NADH + H+
-
 ?
1.1.1.1 1-octanol + NAD+ 11% activity compared to benzyl alcohol Pseudomonas putida octanal + NADH + H+
-
 ?
1.1.1.1 1-pentanol + NAD+ 152% activity compared to benzyl alcohol Pseudomonas putida pentanal + NADH + H+
-
 ?
1.1.1.1 4-nitrobenzyl alcohol + NAD+ 94% activity compared to benzyl alcohol Pseudomonas putida 4-nitrobenzaldehyde + NADH + H+
-
 ?
1.1.1.1 benzaldehyde + NADH + H+ 3% activity compared to benzyl alcohol Pseudomonas putida benzyl alcohol + NAD+
-
 r
1.1.1.1 benzyl alcohol + NAD+ 100% activity Pseudomonas putida benzaldehyde + NADH + H+
-
 r
1.1.1.1 choline + NAD+ 3% activity compared to benzyl alcohol Pseudomonas putida ? + NADH + H+
-
 ?
1.1.1.1 decyl aldehyde + NADH + H+ 21% activity compared to benzyl alcohol Pseudomonas putida 1-decanol + NAD+
-
 ?
1.1.1.1 heptaldehyde + NADH + H+ 33% activity compared to benzyl alcohol Pseudomonas putida ? + NAD+
-
 ?
1.1.1.1 hexaldehyde + NADH + H+ 7% activity compared to benzyl alcohol Pseudomonas putida 1-hexanol + NAD+
-
 ?
1.1.1.1 hydrocinnamaldehyde + NADH + H+ 12% activity compared to benzyl alcohol Pseudomonas putida hydrocinnamyl alcohol + NAD+
-
 r
1.1.1.1 hydrocinnamyl alcohol + NAD+ 31% activity compared to benzyl alcohol Pseudomonas putida hydrocinnamaldehyde + NADH + H+
-
 r
1.1.1.1 additional information no activity towards methanol, ethanol, 1-propanol, triethylene glycol, polyethylene glycol 400, polyethylene glycol 1000, D-sorbitol, D-sorbose, formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and valeraldehyde Pseudomonas putida ?
-
 ?
1.1.1.1 additional information no activity towards methanol, ethanol, 1-propanol, triethylene glycol, polyethylene glycol 400, polyethylene glycol 1000, D-sorbitol, D-sorbose, formaldehyde, acetaldehyde, propionaldehyde, butyraldehyde, and valeraldehyde Pseudomonas putida S-5 ?
-
 ?
1.1.1.1 nonyl aldehyde + NADH + H+ 25% activity compared to benzyl alcohol Pseudomonas putida 1-nonanol + NAD+
-
 ?
1.1.1.1 nonylphenol polyethoxylate 9 + NAD+ 7% activity compared to benzyl alcohol Pseudomonas putida ? + NADH
-
 ?
1.1.1.1 octyl aldehyde + NADH + H+ 29% activity compared to benzyl alcohol Pseudomonas putida 1-octanol + NAD+
-
 ?
1.1.1.1 octylphenol polyethoxylate 2 + NAD+ 59% activity compared to benzyl alcohol Pseudomonas putida ? + NADH
-
 ?
1.1.1.1 octylphenol polyethoxylate 8 + NAD+ 7% activity compared to benzyl alcohol Pseudomonas putida ? + NADH
-
 ?
1.1.1.1 polyoxyethylene 8 decyl ether + NAD+ 12% activity compared to benzyl alcohol Pseudomonas putida ? + NADH
-
 ?
1.1.1.1 Triton X-100 + NAD+ 7% activity compared to benzyl alcohol Pseudomonas putida ? + NADH
-
 ?
1.1.1.1 Triton X-114 + NAD+ 3% activity compared to benzyl alcohol Pseudomonas putida ? + NADH
-
 ?
1.1.1.1 Triton X-165 + NAD+ 24% activity compared to benzyl alcohol Pseudomonas putida ? + NADH
-
 ?
1.1.1.1 Triton X-35 + NAD+ 43% activity compared to benzyl alcohol Pseudomonas putida ? + NADH
-
 ?
1.1.1.1 Triton X-405 + NAD+ 19% activity compared to benzyl alcohol Pseudomonas putida ? + NADH
-
 ?
1.1.1.1 Triton X-45 + NAD+ 7% activity compared to benzyl alcohol Pseudomonas putida ? + NADH
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.1 ADH1
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.1 NAD+
-
 Pseudomonas putida