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Literature summary extracted from
- Production of extracellular exoinulinase from Kluyveromyces marxianus YS-1 using root tubers of Asparagus officinalis (2008), Biores. Technol., 99, 7418-7423.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.7 | inulin | with raw inulin in bioreactor, progressive increase in inulinase production with increase in inulin concentration up to 4.0% with a maximum inulinase activity of 50.2 IU/ml | Kluyveromyces marxianus |  |
| 3.2.1.7 | additional information | inulinase yield in bioreactor is almost 6times higher than basal medium used in shake flask. Tween-60 has no significant effect. Inoculum of 12 h old yeast culture is optimum for inulinase activity (36.9 IU/ml). 5% of 12 h old culture is optimum for the production of inulinase in the medium. Progressive increase in inulinase activity up to 37.0 IU/ml as the agitation speed is increased to 150 rpm. Maximum production of inulinase (40.2 IU/ml) after 60 h of incubation | Kluyveromyces marxianus | |
| 3.2.1.7 | Sodium dodecyl sulfate | 0.001% is most effective for the inulinase activity (36.9 IU/ml) | Kluyveromyces marxianus | |
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.2.1.7 | industry | higher inulinase activity from Kluyveromyces marxianus YS-1 is achieved by root powder extract of Asparagus officinalis. This plant material can be opted as a cheap inulin source for the production of inulinase | Kluyveromyces marxianus |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.7 | Brij-35 | - |
Kluyveromyces marxianus | |
| 3.2.1.7 | Cu2+ | inhibitory on inulinase synthesis | Kluyveromyces marxianus | |
| 3.2.1.7 | Fe3+ | inhibitory on inulinase synthesis | Kluyveromyces marxianus | |
| 3.2.1.7 | inulin | beyond 4% concentration, inulinase yield declines gradually, which may be attributed to the catabolic repression caused by higher inulin concentration | Kluyveromyces marxianus | |
| 3.2.1.7 | additional information | enzyme activity decreases subsequently and reaches to 28.2 IU/mL at pH 7.5. At pH 4.0, the enzyme activity is very low (21.8 IU/ml). At 40°C, enzyme activity decreases to 21.1 IU/ml | Kluyveromyces marxianus | |
| 3.2.1.7 | Sodium dodecyl sulfate | beyond 0.002% it is inhibitory | Kluyveromyces marxianus | |
| 3.2.1.7 | Triton X-100 | - |
Kluyveromyces marxianus | |
| 3.2.1.7 | Tween-20 | - |
Kluyveromyces marxianus | |
| 3.2.1.7 | Tween-80 | - |
Kluyveromyces marxianus | |
| 3.2.1.7 | Zn2+ | inhibitory on inulinase synthesis | Kluyveromyces marxianus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.80 | extracellular | - |
Kluyveromyces marxianus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.7 | Co2+ | 2 mM significantly increases inulinase activity to nearly 110% | Kluyveromyces marxianus | |
| 3.2.1.7 | Mg2+ | 2 mM significantly increases inulinase activity to nearly 114% | Kluyveromyces marxianus | |
| 3.2.1.7 | Mn2+ | 2 mM significantly increases inulinase activity to nearly 125% | Kluyveromyces marxianus | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.7 | Kluyveromyces marxianus | - |
- |
- |
| 3.2.1.7 | Kluyveromyces marxianus Ys-1 | - |
- |
- |
| 3.2.1.80 | Kluyveromyces marxianus | - |
YS-1 | - |
| 3.2.1.80 | Kluyveromyces marxianus Ys-1 | - |
YS-1 | - |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.7 | inulin + H2O | raw inulin from Asparagus officinalis | Kluyveromyces marxianus | ? | - |
? | |
| 3.2.1.7 | inulin + H2O | raw inulin from Asparagus officinalis | Kluyveromyces marxianus Ys-1 | ? | - |
? | |
| 3.2.1.80 | inulin + H2O | root powder extract of Asparagus officinalis. The product mainly consists of fructose and traces of glucose | Kluyveromyces marxianus | D-fructose + ? | - |
? | |
| 3.2.1.80 | inulin + H2O | root powder extract of Asparagus officinalis. The product mainly consists of fructose and traces of glucose | Kluyveromyces marxianus Ys-1 | D-fructose + ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.7 | beta-D-fructan fructanohydrolase | - |
Kluyveromyces marxianus |
| 3.2.1.80 | beta-D-fructan fructanohydrolase | - |
Kluyveromyces marxianus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.7 | 30 | - |
- |
Kluyveromyces marxianus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.7 | 6.5 | - |
- |
Kluyveromyces marxianus |
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