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Literature summary extracted from

  • Han, Y.; Chen, H.
    Characterization of beta-glucosidase from corn stover and its application in simultaneous saccharification and fermentation (2008), Biores. Technol., 99, 6081-6087.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.21 industry application in simultaneous saccharification and fermentation, evaluation of the suitability of plant glycosyl hydrolases in lignocellulose conversion Zea mays

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Ag+
-
 Zea mays
3.2.1.21 cellobiose competitive versus substrate salicin Zea mays
3.2.1.21 Cu2+
-
 Zea mays
3.2.1.21 D-glucose competitive versus substrate 4-nitrophenyl-beta-D-glucopyranoside Zea mays
3.2.1.21 Hg2+
-
 Zea mays

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 2.3
-
 4-nitrophenyl-beta-D-glucopyranoside pH 4.5, 37°C Zea mays
3.2.1.21 4.6
-
 cellobiose pH 4.5, 37°C Zea mays
3.2.1.21 9.3
-
 Salicin pH 4.5, 37°C Zea mays

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 2-mercaptoethanol activates Zea mays
3.2.1.21 Ca2+ activates Zea mays
3.2.1.21 K+ activates Zea mays
3.2.1.21 Mg2+ activates Zea mays
3.2.1.21 additional information no effects by EDTA and NaCl Zea mays

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 62400
-
 native PAGE Zea mays

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Zea mays
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 native enzyme 45fold to homogeneity by ammonium sulfate fractionation, ion exchange chromatography, and gel filtration Zea mays

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 13.5
-
 purified enzyme Zea mays

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Zea mays 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Zea mays 2 beta-D-glucose
-
 ?
3.2.1.21 salicin + H2O
-
 Zea mays beta-D-glucose + 2-(hydroxymethyl)phenol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 62400, SDS-PAGE Zea mays
3.2.1.21 More capillary LC–ESI/MS peptide sequence determination and protein identification Zea mays

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 salicinase
-
 Zea mays

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 37
-
-
 Zea mays

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.21 20 60
-
 Zea mays

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 30 40 stable within this range, rapid decrease in activity below 30°C and above 40°C Zea mays

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 4.8
-
-
 Zea mays

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 3.6 6
-
 Zea mays