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Literature summary extracted from

  • Naeem, A.; Fatima, S.; Khan, R.H.
    Characterization of partially folded intermediates of papain in presence of cationic, anionic, and nonionic detergents at low pH (2006), Biopolymers, 83, 1-10.
    View publication on PubMed

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.4.22.2 hexadecyltrimethyl ammonium bromide the presence of hexadecyltrimethyl ammonium bromide allows 41% recovery of enzymatic activity of acid-unfolded papain in the presence of hexadecyltrimethyl ammonium bromide the enzyme exists as a compact intermediate with regain of native-like secondary and partial tertiary structure as well as high 8-anilino-1-naphthalene-sulfonic acid binding with the partially recovered enzymatic activity Carica papaya
3.4.22.2 SDS the presence of SDS allows 43% recovery of enzymatic activity of acid-unfolded papain, addition of 8 mM SDS results in the loss of 8-anilino-1-naphthalene-sulfonic acid binding sites exhibited by a decrease in 8-anilino-1-naphthalene-sulfonic acid fluorescence intensity, suggesting the burial of hydrophobic patches, papain at low pH and in the presence of SDS exists in a partially folded state characterized by native-like secondary structure and tertiary folds Carica papaya
3.4.22.2 Tween the presence of Tween-20 allows 39% recovery of enzymatic activity of acid-unfolded papain in the presence of Tween-20, acid-unfolded papain exists as a compact intermediate with molten-globule-like characteristics Carica papaya

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.2 Carica papaya
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
3.4.22.2 acid-unfolded papain in the presence of 8 mM SDS and 3.5 mM hexadecyltrimethyl ammonium bromide retains its partial tertiary structure Carica papaya

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.2 latex
-
Carica papaya
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.2 casein + H2O
-
Carica papaya ?
-
?

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.2 2
-
the activity of papain is reduced to 35% at pH 2 and in the absence of detergents, at pH 2 papain exhibits a substantial amount of secondary structure and is relatively less denatured compared with 6 M guanidine hydrochloride but loses the persistent tertiary contacts of the native state Carica papaya