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Literature summary extracted from

  • Yerkes, N.; Wu, J.X.; McCoy, E.; Galan, M.C.; Chen, S.; OConnor, S.E.
    Substrate specificity and diastereoselectivity of strictosidine glucosidase, a key enzyme in monoterpene indole alkaloid biosynthesis (2007), Bioorg. Med. Chem. Lett., 18, 3095-3098.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.105 expression in Escherichia coli Catharanthus roseus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.105 0.071
-
 6-methylstrictosidine pH 6.0, 37°C Catharanthus roseus
3.2.1.105 0.092
-
 7-methylstrictosidine pH 6.0, 37°C Catharanthus roseus
3.2.1.105 0.12
-
 7-fluorostrictosidine pH 6.0, 37°C Catharanthus roseus
3.2.1.105 0.14
-
 8-methylstrictosidine pH 6.0, 37°C Catharanthus roseus
3.2.1.105 0.15
-
 6-fluorostrictosidine pH 6.0, 37°C Catharanthus roseus
3.2.1.105 0.22
-
 strictosidine pH 6.0, 37°C Catharanthus roseus
3.2.1.105 0.22
-
 5-methylstrictosidine pH 6.0, 37°C Catharanthus roseus
3.2.1.105 0.37
-
 6-methoxystrictosidine pH 6.0, 37°C Catharanthus roseus
3.2.1.105 0.43
-
 7-methoxystrictosidine pH 6.0, 37°C Catharanthus roseus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.105 Catharanthus roseus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.105 5-methylstrictosidine + H2O
-
 Catharanthus roseus D-glucose + 5-methylstrictosidine aglycone
-
 ?
3.2.1.105 6-fluorostrictosidine + H2O
-
 Catharanthus roseus D-glucose + 6-fluorostrictosidine aglycone
-
 ?
3.2.1.105 6-methoxystrictosidine + H2O
-
 Catharanthus roseus D-glucose + 6-methoxystrictosidine aglycone
-
 ?
3.2.1.105 6-methylstrictosidine + H2O
-
 Catharanthus roseus D-glucose + 6-methylstrictosidine aglycone
-
 ?
3.2.1.105 7-fluorostrictosidine + H2O
-
 Catharanthus roseus D-glucose + 7-fluorostrictosidine aglycone
-
 ?
3.2.1.105 7-methoxystrictosidine + H2O
-
 Catharanthus roseus D-glucose + 7-methoxystrictosidine aglycone
-
 ?
3.2.1.105 7-methylstrictosidine + H2O
-
 Catharanthus roseus D-glucose + 7-methylstrictosidine aglycone
-
 ?
3.2.1.105 8-methylstrictosidine + H2O
-
 Catharanthus roseus D-glucose + 8-methylstrictosidine aglycone
-
 ?
3.2.1.105 strictosidine + H2O
-
 Catharanthus roseus D-glucose + strictosidine aglycone
-
 ?
3.2.1.105 vincoside + H2O
-
 Catharanthus roseus D-glucose + vincoside aglycone
-
 ?
3.2.1.105 vincoside pent-4-yn-1-yl ester + H2O
-
 Catharanthus roseus D-glucose + vincoside pent-4-yn-1-yl ester aglycone
-
 ?